Prbbbb:Coomassi Ethanol stain

From OpenWetWare
Revision as of 04:19, 6 August 2009 by Raik (talk | contribs) (New page: Ethanol based variant of the Coomassi protein stain solution. ==Mix== * 40 % Ethanol * 7 % Acetic Acid * 0.1 % w/v Coomassi Brilliant Blue-R * double-distilled water ==Usage== # Stain...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Ethanol based variant of the Coomassi protein stain solution.

Mix

  • 40 % Ethanol
  • 7 % Acetic Acid
  • 0.1 % w/v Coomassi Brilliant Blue-R
  • double-distilled water

Usage

  1. Stain at room temperature for 1h or until overnight
  2. De-stain 30 min with Prbbbb:Coomassi Ethanol destain

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


References

Contact

or instead, discuss this protocol.