Preparing chemically competent cells

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*Grow a 5-25mL culture to an OD<sub>600</sub> of 0.2 <math>\rightarrow</math> 0.5
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*Centrifuge for 10 minutes at 3000rpm and <math>4^o</math>C.
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*Remove supernatant and replace with 10% original volume TSS.
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*Make 100uL aliquots and store at <math>-80^o</math>C.
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[[Category:E.Coli Protocol]]
[[Category:E.Coli Protocol]]

Revision as of 08:46, 24 May 2005

  • Grow a 5-25mL culture to an OD600 of 0.2 \rightarrow 0.5
  • Centrifuge for 10 minutes at 3000rpm and 4oC.
  • Remove supernatant and replace with 10% original volume TSS.
  • Make 100uL aliquots and store at − 80oC.
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