Prince:BYU Lipidomics Team

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Basic Protocol

• Get sample from almost any source
• Extraction
  • use Kaluzne (both methods use Folch reagent [2:1 chloroform:methanol])
  • trying to implement Bligh & Dyer (gold standard)
• Mass Spectrometry
  • ESI-Agilent TOF
  • Tandem MS-Q-Star (8-10am)
• Analysis
  • Matlab (particular toolbox?)

main analytes are (+) phosphatidylcholine** and phosphatidylethanolamine analytes in (-) mode would be phosphatidylserines and phosphatidylethanolamines (expected to have stronger signal in - mode).

FAME-GC associated with Craig Thulin and UVU. Mary can prepare these and knows who has this instrument.

Group Members

• Matthew Linford
• John Prince
• Dean Flanders
• Jennifer MacDonald
• Craig Thulin
• Mary Blackburn
• Mackay Merrill
• Landon Weist
• Jonathan Lee

Relevant Publications

1. Dang L, White DW, Gross S, Bennett BD, Bittinger MA, Driggers EM, Fantin VR, Jang HG, Jin S, Keenan MC, Marks KM, Prins RM, Ward PS, Yen KE, Liau LM, Rabinowitz JD, Cantley LC, Thompson CB, Vander Heiden MG, and Su SM. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature. 2009 Dec 10;462(7274):739-44. DOI:10.1038/nature08617 |

   This has a great protocol for negative ion mode exctraction and analysis on the Orbitrap.