Prince:BYU Lipidomics Team

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Contents

Basic Protocol

  • Get sample from almost any source
  • Extraction (both methods use Folch reagent [2:1 chloroform:methanol])
    • use Kaluzne method
    • trying to implement Bligh & Dyer (gold standard)
    • MTBE Extraction [1]
  • Mass Spectrometry
    • ESI-Agilent TOF
    • Tandem MS-Q-Star (8-10am)
  • Analysis
    • Matlab (particular toolbox?) PCA analysis

Main analytes are phosphatidylcholine and phosphatidylethanolamine. Analytes in (-) mode would be phosphatidylserines and phosphatidylethanolamines (which are expected to have stronger signal in - mode). Typical number of analytes?

FAME-GC associated with Craig Thulin and UVU.

Literature

Lipid Signalling

Sphingolipid signalling. [2]

Lipid mediators in health and disease. [3]

Lipid mediators for inflammation resolution. [4]

Mass Spectrometry

Practical essentials of ESI.* [5]

Solid tutorial review on mass spec "-omics."* [6]

Recent advances (2011) in MS lipidomics. [7]

General aspects of lipidomics. [8]

Basics of ESI and intrasource separation shotgun lipidomics.** [9]

Tissue sample preparation for intrasource separation.** [10]

AMDMS-SL. [11]

Top-down lipidomic strategy.** [12]

Blood plasma and top-down lipidomics.* [13]

Yeast quantitative shotgun lipidomics. [14] [15]

Eicosanoid lipidomics. [16]

Bioactive lipidomics. [17]

Lipid mediator informatics-lipidomics. [18]

Mediator lipidomics. [19]

Bioinformatics

Chemometrics. [20] [21]

S-plot. [22]

PCA package. [23]

K-OPLS package. [24]

Molecular fragmentation query language. [25]

Special Journal Issues on Lipidomics

Journal of Chromatography B [26]

Prostaglandins and Other Lipid Mediators [27]

European Journal of Lipid Science and Technology [28]

Group Members

Current

  • Mary Blackburn
  • Mackay Merrill
  • Dean Flanders
  • Landon Weist
  • Rob Roden
  • Greg Jackson
  • Daniel Edmondson
  • Matthew Linford
  • Craig Thulin
  • Steven Wood
  • John Prince

Former or Associated

  • Jennifer MacDonald
  • Jonathan Lee
  • Vinod Chaudhary
  • Steven Herron

Relevant Publications

Error fetching PMID 19935646:
  1. Error fetching PMID 19935646: [P1]
    This has a great protocol for negative ion mode extraction and analysis on the Orbitrap.

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