Prince:Mass Spectrometry

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(Added a link to the intro page I'm about to write.)
(UPLC)
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===UPLC===
===UPLC===
* [[Prince:UPLC|UPLC]] (Fittings/tubing/flow rates)
* [[Prince:UPLC|UPLC]] (Fittings/tubing/flow rates)
 +
** 10 micron needles provide best nano-spray at 200-300 nl/min
===Tuning/Calibration===
===Tuning/Calibration===

Revision as of 15:42, 22 November 2010

Image:Prince lab logo.png

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Contents

New Users

We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you.

Introduction to Mass Spectrometry

Sample Preparation

Proven Methods:

Side Notes:

Equipment

Gases

[From the wall out]

  • Helium UHP [LTQ] (40psi)
  • Nitrogen UHP [HCD] (100psi)
  • Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
  • Nitrogen? Normal? psi? [for UPLC]

UPLC

  • UPLC (Fittings/tubing/flow rates)
    • 10 micron needles provide best nano-spray at 200-300 nl/min

Tuning/Calibration

Mascot Restart

 user@jp1:~$ ls /etc/init.d/mascot
/etc/init.d/mascot
user@jp1:~$ sudo /etc/init.d/mascot status
[sudo] password for user:
user@jp1:~$ sudo /etc/init.d/mascot restart
 * Mascot monitor is not running
 * Mascot monitor started
user@jp1:~$
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