Prince:Mass Spectrometry

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(Sample Preparation)
(Sample Preparation)
Line 10: Line 10:
* [[Prince:FASP|FASP]] (filter aided sample preparation)
* [[Prince:FASP|FASP]] (filter aided sample preparation)
* [[Prince:In Gel FASP|In Gel FASP]]
* [[Prince:In Gel FASP|In Gel FASP]]
-
* [[Prince:Large Scale Yeast FASP|:Large Scale Yeast FASP]]
+
* [[Prince:Yeast FASP|Yeast FASP]]
'''Side Notes:'''
'''Side Notes:'''
* [[Prince:Reduction & Alkylation|Reduction & Alkylation]]
* [[Prince:Reduction & Alkylation|Reduction & Alkylation]]

Revision as of 08:18, 13 September 2012

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Contents

New Users

We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you.

Introduction to Mass Spectrometry

Sample Preparation

Proven Methods:

Side Notes:

Wet Lab Rules

Equipment

Gases

[From the wall out]

  • Helium UHP [LTQ] (40psi)
  • Nitrogen UHP [HCD] (100psi)
  • Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
  • Nitrogen Normal (100psi) [for UPLC]

UPLC

Tuning/Calibration

Mascot Restart

 user@jp1:~$ ls /etc/init.d/mascot
/etc/init.d/mascot
user@jp1:~$ sudo /etc/init.d/mascot status
[sudo] password for user:
user@jp1:~$ sudo /etc/init.d/mascot restart
 * Mascot monitor is not running
 * Mascot monitor started
user@jp1:~$

Scheduling

You may access the scheduling site here:

Orbitrap Scheduling

Personal tools