Prince:Mass Spectrometry

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===Sample Preparation===
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{{PrinceLab}}
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===New Users===
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We recommend you peruse this site and become more familiar with the content.  Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you.
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[[Prince:Introduction|Introduction to Mass Spectrometry]]
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===Sample Preparation===
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'''Proven Methods:'''
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* [[Prince:FASP|FASP]] (filter aided sample preparation)
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* [[Prince:In Gel FASP|In Gel FASP]]
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* [[Prince:Yeast FASP|Yeast FASP]]
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'''Side Notes:'''
* [[Prince:Reduction & Alkylation|Reduction & Alkylation]]
* [[Prince:Reduction & Alkylation|Reduction & Alkylation]]
* [[Prince:Microwave Digestion|Microwave Digestion]]
* [[Prince:Microwave Digestion|Microwave Digestion]]
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* [[Prince:FASP|FASP]] (filter aided sample preparation)
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====Wet Lab Rules====
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* [[Prince:Prince Lab Practices| Lab Practices]]
===Equipment===
===Equipment===
* [[Prince:Tech Support|Tech Support]]
* [[Prince:Tech Support|Tech Support]]
* [[Prince:MS Prep Consumables|MS Prep Consumables]]
* [[Prince:MS Prep Consumables|MS Prep Consumables]]
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* [[Prince::MS Consumables|MS Consumables]]
* [[Prince:Miscellaneous Tips|Miscellaneous Tips]]
* [[Prince:Miscellaneous Tips|Miscellaneous Tips]]
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===Gases===
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[From the wall out]
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* Helium UHP [LTQ] (40psi)
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* Nitrogen UHP [HCD] (100psi)
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* Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
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* Nitrogen Normal (100psi) [for UPLC]
===UPLC===
===UPLC===
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*[[Prince:Troubleshooting_the_Eksigent_UPLC_system|Troubleshooting the Eksigent UPLC system]]
* [[Prince:UPLC|UPLC]] (Fittings/tubing/flow rates)
* [[Prince:UPLC|UPLC]] (Fittings/tubing/flow rates)
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** 10 micron needles provide best nano-spray at 200-300 nl/min
===Tuning/Calibration===
===Tuning/Calibration===
* [[Prince:Tuning and Calibration|Tuning and Calibration]]
* [[Prince:Tuning and Calibration|Tuning and Calibration]]
* [[Prince:Klaus's Cleaning Mix|Klaus's Cleaning Mix]]
* [[Prince:Klaus's Cleaning Mix|Klaus's Cleaning Mix]]
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===Mascot Restart===
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<pre> user@jp1:~$ ls /etc/init.d/mascot
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/etc/init.d/mascot
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user@jp1:~$ sudo /etc/init.d/mascot status
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[sudo] password for user:
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user@jp1:~$ sudo /etc/init.d/mascot restart
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* Mascot monitor is not running
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* Mascot monitor started
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user@jp1:~$</pre>
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You may also try this.
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If the mascot daemon is not running:
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[You need to have sudo priviledges on jp1]
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In the command line enter;
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'''sudo /mascot/bin/ms-monitor.exe'''
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From home, this could be done, after vpn-ing into the chemistry network with a single ssh command:
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'''ssh -t <USERNAME>@jp1.chem.byu.edu 'sudo /mascot/bin/ms-monitor.exe''''
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=Scheduling=
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You may access the scheduling site here:
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[http://mass-spec-scheduler.heroku.com/ Orbitrap Scheduling]

Current revision

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Contents

New Users

We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you.

Introduction to Mass Spectrometry

Sample Preparation

Proven Methods:

Side Notes:

Wet Lab Rules

Equipment

Gases

[From the wall out]

  • Helium UHP [LTQ] (40psi)
  • Nitrogen UHP [HCD] (100psi)
  • Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
  • Nitrogen Normal (100psi) [for UPLC]

UPLC

Tuning/Calibration

Mascot Restart

 user@jp1:~$ ls /etc/init.d/mascot
/etc/init.d/mascot
user@jp1:~$ sudo /etc/init.d/mascot status
[sudo] password for user:
user@jp1:~$ sudo /etc/init.d/mascot restart
 * Mascot monitor is not running
 * Mascot monitor started
user@jp1:~$

You may also try this.

If the mascot daemon is not running:

[You need to have sudo priviledges on jp1]

In the command line enter;

sudo /mascot/bin/ms-monitor.exe


From home, this could be done, after vpn-ing into the chemistry network with a single ssh command:

ssh -t <USERNAME>@jp1.chem.byu.edu 'sudo /mascot/bin/ms-monitor.exe'

Scheduling

You may access the scheduling site here:

Orbitrap Scheduling

Personal tools