Prince:Mass Spectrometry: Difference between revisions
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{{PrinceLab}} | {{PrinceLab}} | ||
===New Users=== | |||
We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you. | |||
[[Prince:Introduction|Introduction to Mass Spectrometry]] | |||
===Sample Preparation=== | ===Sample Preparation=== | ||
| Line 5: | Line 10: | ||
* [[Prince:FASP|FASP]] (filter aided sample preparation) | * [[Prince:FASP|FASP]] (filter aided sample preparation) | ||
* [[Prince:In Gel FASP|In Gel FASP]] | * [[Prince:In Gel FASP|In Gel FASP]] | ||
* [[Prince:Yeast FASP|Yeast FASP]] | |||
'''Side Notes:''' | '''Side Notes:''' | ||
* [[Prince:Reduction & Alkylation|Reduction & Alkylation]] | * [[Prince:Reduction & Alkylation|Reduction & Alkylation]] | ||
* [[Prince:Microwave Digestion|Microwave Digestion]] | * [[Prince:Microwave Digestion|Microwave Digestion]] | ||
====Wet Lab Rules==== | |||
* [[Prince:Prince Lab Practices| Lab Practices]] | |||
===Equipment=== | ===Equipment=== | ||
* [[Prince:Tech Support|Tech Support]] | * [[Prince:Tech Support|Tech Support]] | ||
* [[Prince:MS Prep Consumables|MS Prep Consumables]] | * [[Prince:MS Prep Consumables|MS Prep Consumables]] | ||
* [[Prince::MS Consumables|MS Consumables]] | |||
* [[Prince:Miscellaneous Tips|Miscellaneous Tips]] | * [[Prince:Miscellaneous Tips|Miscellaneous Tips]] | ||
| Line 18: | Line 28: | ||
* Nitrogen UHP [HCD] (100psi) | * Nitrogen UHP [HCD] (100psi) | ||
* Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi) | * Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi) | ||
* Nitrogen | * Nitrogen Normal (100psi) [for UPLC] | ||
===UPLC=== | ===UPLC=== | ||
*[[Prince:Troubleshooting_the_Eksigent_UPLC_system|Troubleshooting the Eksigent UPLC system]] | |||
* [[Prince:UPLC|UPLC]] (Fittings/tubing/flow rates) | * [[Prince:UPLC|UPLC]] (Fittings/tubing/flow rates) | ||
** 10 micron needles provide best nano-spray at 200-300 nl/min | |||
===Tuning/Calibration=== | ===Tuning/Calibration=== | ||
| Line 36: | Line 48: | ||
* Mascot monitor started | * Mascot monitor started | ||
user@jp1:~$</pre> | user@jp1:~$</pre> | ||
You may also try this. | |||
If the mascot daemon is not running: | |||
[You need to have sudo priviledges on jp1] | |||
In the command line enter; | |||
'''sudo /mascot/bin/ms-monitor.exe''' | |||
From home, this could be done, after vpn-ing into the chemistry network with a single ssh command: | |||
'''ssh -t <USERNAME>@jp1.chem.byu.edu 'sudo /mascot/bin/ms-monitor.exe'''' | |||
=Scheduling= | |||
You may access the scheduling site here: | |||
[http://mass-spec-scheduler.heroku.com/ Orbitrap Scheduling] | |||
Latest revision as of 16:01, 1 February 2013
New Users
We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you.
Introduction to Mass Spectrometry
Sample Preparation
Proven Methods:
- FASP (filter aided sample preparation)
- In Gel FASP
- Yeast FASP
Side Notes:
Wet Lab Rules
Equipment
Gases
[From the wall out]
- Helium UHP [LTQ] (40psi)
- Nitrogen UHP [HCD] (100psi)
- Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
- Nitrogen Normal (100psi) [for UPLC]
UPLC
- Troubleshooting the Eksigent UPLC system
- UPLC (Fittings/tubing/flow rates)
- 10 micron needles provide best nano-spray at 200-300 nl/min
Tuning/Calibration
Mascot Restart
user@jp1:~$ ls /etc/init.d/mascot /etc/init.d/mascot user@jp1:~$ sudo /etc/init.d/mascot status [sudo] password for user: user@jp1:~$ sudo /etc/init.d/mascot restart * Mascot monitor is not running * Mascot monitor started user@jp1:~$
You may also try this.
If the mascot daemon is not running:
[You need to have sudo priviledges on jp1]
In the command line enter;
sudo /mascot/bin/ms-monitor.exe
From home, this could be done, after vpn-ing into the chemistry network with a single ssh command:
ssh -t <USERNAME>@jp1.chem.byu.edu 'sudo /mascot/bin/ms-monitor.exe'
Scheduling
You may access the scheduling site here:
