Prince:Mass Spectrometry: Difference between revisions
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===New Users=== | |||
We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you. | |||
[[Prince:Introduction|Introduction to Mass Spectrometry]] | |||
===Sample Preparation=== | ===Sample Preparation=== |
Revision as of 08:38, 22 November 2010
New Users
We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you.
Introduction to Mass Spectrometry
Sample Preparation
Proven Methods:
- FASP (filter aided sample preparation)
- In Gel FASP
Side Notes:
Equipment
Gases
[From the wall out]
- Helium UHP [LTQ] (40psi)
- Nitrogen UHP [HCD] (100psi)
- Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
- Nitrogen? Normal? psi? [for UPLC]
UPLC
- UPLC (Fittings/tubing/flow rates)
Tuning/Calibration
Mascot Restart
user@jp1:~$ ls /etc/init.d/mascot /etc/init.d/mascot user@jp1:~$ sudo /etc/init.d/mascot status [sudo] password for user: user@jp1:~$ sudo /etc/init.d/mascot restart * Mascot monitor is not running * Mascot monitor started user@jp1:~$