Prince:Miscellaneous Tips: Difference between revisions
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(New page: * Excess iodoacetamide can cause primary amine N-terminal carbamidomethylation of peptides. [http://pubs.acs.org/doi/abs/10.1021/ac0103423 see here] * Iodoacetamide can cause conflict with...) |
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* Excess iodoacetamide can cause primary amine N-terminal carbamidomethylation of peptides. [http://pubs.acs.org/doi/abs/10.1021/ac0103423 see here] | * Excess iodoacetamide can cause primary amine N-terminal carbamidomethylation of peptides. [http://pubs.acs.org/doi/abs/10.1021/ac0103423 see here] | ||
* Iodoacetamide can cause conflict with Ubiquitin GG patterns, but this side-effect is eliminated when the reaction is performed at 21 °C or cooler. ([http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4W03PJK-J&_user=456938&_coverDate=04%2F03%2F2009&_rdoc=17&_fmt=high&_orig=browse&_srch=doc-info%28%23toc%237051%232009%23998629998%231025049%23FLA%23display%23Volume%29&_cdi=7051&_sort=d&_docanchor=&view=c&_ct=21&_acct=C000021830&_version=1&_urlVersion=0&_userid=456938&md5=e0edfa405593a2d13a73eb825e535a0a article] | [http://www.sciencedirect.com/science/MiamiMultiMediaURL/B6WSN-4W03PJK-J/B6WSN-4W03PJK-J-1/7051/html/S0092867409000890/c96f3db030443af5651cfffbcf6c92dd/mmc1.pdf relevant supplementary data]) | * Iodoacetamide can cause conflict with Ubiquitin GG patterns, but this side-effect is eliminated when the reaction is performed at 21 °C or cooler. ([http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4W03PJK-J&_user=456938&_coverDate=04%2F03%2F2009&_rdoc=17&_fmt=high&_orig=browse&_srch=doc-info%28%23toc%237051%232009%23998629998%231025049%23FLA%23display%23Volume%29&_cdi=7051&_sort=d&_docanchor=&view=c&_ct=21&_acct=C000021830&_version=1&_urlVersion=0&_userid=456938&md5=e0edfa405593a2d13a73eb825e535a0a article] | [http://www.sciencedirect.com/science/MiamiMultiMediaURL/B6WSN-4W03PJK-J/B6WSN-4W03PJK-J-1/7051/html/S0092867409000890/c96f3db030443af5651cfffbcf6c92dd/mmc1.pdf relevant supplementary data]) | ||
* It is thought that formic acid in acetonitrile causes polymerization and contribute to plugging of tips and the HPLC. One can prevent this by making fresh ACN/formic acid mixes weekly, or just use straight ACN (no formic acid). The water and formic acid is OK. [Reference to the ABRF discussion on this??] | |||
Revision as of 10:25, 2 June 2010
- Excess iodoacetamide can cause primary amine N-terminal carbamidomethylation of peptides. see here
- Iodoacetamide can cause conflict with Ubiquitin GG patterns, but this side-effect is eliminated when the reaction is performed at 21 °C or cooler. (article | relevant supplementary data)
- It is thought that formic acid in acetonitrile causes polymerization and contribute to plugging of tips and the HPLC. One can prevent this by making fresh ACN/formic acid mixes weekly, or just use straight ACN (no formic acid). The water and formic acid is OK. [Reference to the ABRF discussion on this??]
