Prince:Proteomics: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 5: | Line 5: | ||
=Experiment Design= | =Experiment Design= | ||
[[image: | [[image:sample prep work flow.jpeg|500px|]] | ||
[[Prince:Cell Lysis|Cell Lysis]] | [[Prince:Cell Lysis|Cell Lysis]] |
Revision as of 13:02, 3 February 2012
Introduction
- Proteomics is the study of protein structure and function. Mass spectrometry measures the m/z (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the m/z ratio of proteins or pieces of proteins called peptides. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation of proteins in the PAS kinase network as a result of PAS kinase perturbation.
Experiment Design
FASP (Filter-aided Sample Preparation)