Prince:Proteomics: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 5: Line 5:
=Experiment Design=
=Experiment Design=


[[image:Sample prep work flow.jpeg|500px|]]
[[image:work flow.jpeg|500px|]]


[[Prince:Cell Lysis|Cell Lysis]]
[[Prince:Cell Lysis|Cell Lysis]]

Revision as of 13:05, 3 February 2012

Introduction

Proteomics is the study of protein structure and function. Mass spectrometry measures the m/z (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the m/z ratio of proteins or pieces of proteins called peptides. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation of proteins in the PAS kinase network as a result of PAS kinase perturbation.

Experiment Design

Cell Lysis

FASP (Filter-aided Sample Preparation)

Phospho-protein Enrichment

Mass Spectrometry

Protein Concentration

Results

Yeast Proteome Coverage

UGP1 Coverage

PASK Coverage