Prince:Proteomics: Difference between revisions
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=Introduction= | =Introduction= | ||
: [http://en.wikipedia.org/wiki/Proteomics Proteomics] is the study of [http://en.wikipedia.org/wiki/Protein protein] structure and function. [http://en.wikipedia.org/wiki/Mass_spectrometry Mass spectrometry] measures the ''m/z'' (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the ''m/z'' ratio of proteins or pieces of proteins called [http://en.wikipedia.org/wiki/Peptide peptides]. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation | : [http://en.wikipedia.org/wiki/Proteomics Proteomics] is the study of [http://en.wikipedia.org/wiki/Protein protein] structure and function. [http://en.wikipedia.org/wiki/Mass_spectrometry Mass spectrometry] measures the ''m/z'' (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the ''m/z'' ratio of proteins or pieces of proteins called [http://en.wikipedia.org/wiki/Peptide peptides]. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation in the PAS kinase network as a result of PAS kinase perturbation. | ||
=Experiment Design= | =Experiment Design= | ||
[[image: | [[image:work flow.jpeg|500px|]] | ||
[[Prince:Cell Lysis|Cell Lysis]] | [[Prince:Cell Lysis|Cell Lysis]] | ||
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[[Prince:Filter-aided Sample Preparation|FASP (Filter-aided Sample Preparation)]] | [[Prince:Filter-aided Sample Preparation|FASP (Filter-aided Sample Preparation)]] | ||
[[Prince: | [[Prince:Strong Cation Exchange| SCX (Strong Cation Exchange)]] | ||
[[Prince:Phosphopeptide Enrichment|Phosphopeptide Enrichment]] | |||
[[Prince:Mass Spec|Mass Spectrometry]] | [[Prince:Mass Spec|Mass Spectrometry]] | ||
[[Prince:Protein | [[Prince:Protein Quantitation|Protein Quantitation]] | ||
=Results= | =Results= | ||
Latest revision as of 13:51, 4 February 2012
Introduction
- Proteomics is the study of protein structure and function. Mass spectrometry measures the m/z (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the m/z ratio of proteins or pieces of proteins called peptides. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation in the PAS kinase network as a result of PAS kinase perturbation.
Experiment Design
FASP (Filter-aided Sample Preparation)
