Prince:Proteomics

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(Experiment Design)
(Introduction)
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=Introduction=
=Introduction=
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: [http://en.wikipedia.org/wiki/Proteomics Proteomics] is the study of [http://en.wikipedia.org/wiki/Protein protein] structure and function. [http://en.wikipedia.org/wiki/Mass_spectrometry Mass spectrometry] measures the ''m/z'' (mass to charge) ratio of particles.  So mass spectrometry based proteomics is the study of protein structure and function by identifying the ''m/z'' ratio of proteins or pieces of proteins called [http://en.wikipedia.org/wiki/Peptide peptides].  Mass spectrometry can identify thousands of proteins in a single analysis.  Our goal is to identify and quantitate changes in protein concentration and phosphorylation of proteins in the PAS kinase network as a result of PAS kinase perturbation.
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: [http://en.wikipedia.org/wiki/Proteomics Proteomics] is the study of [http://en.wikipedia.org/wiki/Protein protein] structure and function. [http://en.wikipedia.org/wiki/Mass_spectrometry Mass spectrometry] measures the ''m/z'' (mass to charge) ratio of particles.  So mass spectrometry based proteomics is the study of protein structure and function by identifying the ''m/z'' ratio of proteins or pieces of proteins called [http://en.wikipedia.org/wiki/Peptide peptides].  Mass spectrometry can identify thousands of proteins in a single analysis.  Our goal is to identify and quantitate changes in protein concentration and phosphorylation in the PAS kinase network as a result of PAS kinase perturbation.
=Experiment Design=
=Experiment Design=

Revision as of 15:39, 4 February 2012

Introduction

Proteomics is the study of protein structure and function. Mass spectrometry measures the m/z (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the m/z ratio of proteins or pieces of proteins called peptides. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation in the PAS kinase network as a result of PAS kinase perturbation.

Experiment Design

Cell Lysis

FASP (Filter-aided Sample Preparation)

SCX (Strong Cation Exchange)

Phospho-protein Enrichment

Mass Spectrometry

Protein Concentration

Results

Yeast Proteome Coverage

UGP1 Coverage

PASK Coverage

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