Prince:Proteomics: Difference between revisions
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[[Prince:Mass Spec|Mass Spectrometry]] | [[Prince:Mass Spec|Mass Spectrometry]] | ||
[[Prince:Protein | [[Prince:Protein Quantitation|Protein Quantitation]] | ||
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Revision as of 13:50, 4 February 2012
Introduction
- Proteomics is the study of protein structure and function. Mass spectrometry measures the m/z (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the m/z ratio of proteins or pieces of proteins called peptides. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation in the PAS kinase network as a result of PAS kinase perturbation.
Experiment Design
FASP (Filter-aided Sample Preparation)
