Prince:Proteomics

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Experiment Design)
Current revision (15:51, 4 February 2012) (view source)
(Experiment Design)
 
Line 13: Line 13:
[[Prince:Strong Cation Exchange| SCX (Strong Cation Exchange)]]
[[Prince:Strong Cation Exchange| SCX (Strong Cation Exchange)]]
-
[[Prince:Phospho-protein Enrichment|Phospho-protein Enrichment]]
+
[[Prince:Phosphopeptide Enrichment|Phosphopeptide Enrichment]]
[[Prince:Mass Spec|Mass Spectrometry]]
[[Prince:Mass Spec|Mass Spectrometry]]

Current revision

Introduction

Proteomics is the study of protein structure and function. Mass spectrometry measures the m/z (mass to charge) ratio of particles. So mass spectrometry based proteomics is the study of protein structure and function by identifying the m/z ratio of proteins or pieces of proteins called peptides. Mass spectrometry can identify thousands of proteins in a single analysis. Our goal is to identify and quantitate changes in protein concentration and phosphorylation in the PAS kinase network as a result of PAS kinase perturbation.

Experiment Design

Cell Lysis

FASP (Filter-aided Sample Preparation)

SCX (Strong Cation Exchange)

Phosphopeptide Enrichment

Mass Spectrometry

Protein Quantitation

Results

Yeast Proteome Coverage

UGP1 Coverage

PASK Coverage

Personal tools