Prince:Ultra High Performance Liquid Chromatography: Difference between revisions
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=Brief Introduction= | =Brief Introduction= | ||
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[http://en.wikipedia.org/wiki/Chromatography Chromatography] separates compounds based on compound properties. For instance, size exclusion chromatography separates compounds based on molecular weight and [http://en.wikipedia.org/wiki/Stokes_radius stokes radius]. There are generally two phases in chromatography, the stationary phase and the mobile phase. In column chromatography, the column is made of the stationary phase and the mobile phase pushes molecules through the column. Molecules with a high affinity for the stationary phase are trapped, but molecules with a low affinity for the stationary phase pass through the column in the mobile phase. The gradient, or composition, of the mobile phase during sample loading onto the column has very low column affinity causing the majority of molecules to bind to the column. After loading, one can elute the column in fractions (groups of molecules with similar properties) by increasing the affinity of the mobile phase for the column because the mobile phase competes with the compounds of interest for binding to the stationary phase. Ultra high performance liquid chromatography uses basic chromatography principles, but at a very high pressure and very low flow rate. | [http://en.wikipedia.org/wiki/Chromatography Chromatography] separates compounds based on compound properties. For instance, size exclusion chromatography separates compounds based on molecular weight and [http://en.wikipedia.org/wiki/Stokes_radius stokes radius]. There are generally two phases in chromatography, the stationary phase and the mobile phase. In column chromatography, the column is made of the stationary phase and the mobile phase pushes molecules through the column. Molecules with a high affinity for the stationary phase are trapped, but molecules with a low affinity for the stationary phase pass through the column in the mobile phase. The gradient, or composition, of the mobile phase during sample loading onto the column has very low column affinity causing the majority of molecules to bind to the column. After loading, one can elute the column in fractions (groups of molecules with similar properties) by increasing the affinity of the mobile phase for the column because the mobile phase competes with the compounds of interest for binding to the stationary phase. Ultra high performance liquid chromatography uses basic chromatography principles, but at a very high pressure and very low flow rate. | ||
Revision as of 16:19, 13 February 2012
Brief Introduction
Chromatography separates compounds based on compound properties. For instance, size exclusion chromatography separates compounds based on molecular weight and stokes radius. There are generally two phases in chromatography, the stationary phase and the mobile phase. In column chromatography, the column is made of the stationary phase and the mobile phase pushes molecules through the column. Molecules with a high affinity for the stationary phase are trapped, but molecules with a low affinity for the stationary phase pass through the column in the mobile phase. The gradient, or composition, of the mobile phase during sample loading onto the column has very low column affinity causing the majority of molecules to bind to the column. After loading, one can elute the column in fractions (groups of molecules with similar properties) by increasing the affinity of the mobile phase for the column because the mobile phase competes with the compounds of interest for binding to the stationary phase. Ultra high performance liquid chromatography uses basic chromatography principles, but at a very high pressure and very low flow rate.
