Prince:Ultra High Performance Liquid Chromatography

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(New page: =Brief Introduction= Let's start by just talking about [http://en.wikipedia.org/wiki/Chromatography chromatography]. Chromatography separates compounds based on compound properties. Fo...)
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=Brief Introduction=
=Brief Introduction=
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Let's start by just talking about [http://en.wikipedia.org/wiki/Chromatography chromatography].  Chromatography separates compounds based on compound properties.  For instance, size exclusion chromatography separates compounds based on molecular weight and [http://en.wikipedia.org/wiki/Stokes_radius stokes radius].
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[http://en.wikipedia.org/wiki/Chromatography chromatography] separates compounds based on compound properties.  For instance, size exclusion chromatography separates compounds based on molecular weight and [http://en.wikipedia.org/wiki/Stokes_radius stokes radius].  There are generally two phases in chromatography, the stationary phase and the mobile phase.  In column chromatography, the column is made of the stationary phase and the mobile phase pushes molecules through the column.  Molecules with a high affinity for the stationary phase are trapped, but molecules with a low affinity for the stationary phase pass through the column in the mobile phase.  The gradient, or composition, of the mobile phase during the sample loading onto the column has very low affinity for the column causing the majority of molecules to bind to the column.  After loading, one can elute the column in fractions (groups of molecules with similar properties) by increasing the affinity of the mobile phase for the column because the mobile phase competes with the compounds of interest for binding to the stationary phase.

Revision as of 17:41, 13 February 2012

Brief Introduction

chromatography separates compounds based on compound properties. For instance, size exclusion chromatography separates compounds based on molecular weight and stokes radius. There are generally two phases in chromatography, the stationary phase and the mobile phase. In column chromatography, the column is made of the stationary phase and the mobile phase pushes molecules through the column. Molecules with a high affinity for the stationary phase are trapped, but molecules with a low affinity for the stationary phase pass through the column in the mobile phase. The gradient, or composition, of the mobile phase during the sample loading onto the column has very low affinity for the column causing the majority of molecules to bind to the column. After loading, one can elute the column in fractions (groups of molecules with similar properties) by increasing the affinity of the mobile phase for the column because the mobile phase competes with the compounds of interest for binding to the stationary phase.

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