Protein
From OpenWetWare
General pages
- Wikiomics:Nuclear Magnetic Resonance
- Wikiomics:RMSD
- Wikiomics:Searching for 3D functional sites in a protein structure
- Wikiomics:Structural bioinformatics
- Wikiomics:X-ray crystallography
- Moore:Working with coli
- Protein-protein interaction databases
Protocols
- Beta-galactosidase assay
- Modeller
- Acrylamide gels
- Digital Tools & Resources
- Silver staining
- Purification of His-tagged proteins
- BLAST
- Free Sulfhydryl Determination
- Homology Modelling
- Coot and WinCoot for X-ray Crystallography Model Building
- Antigen retrieval
- Electrophoretic mobility shift assay
- Protein blot (Western) hub
- Chromatin Immunoprecipitation
- Protein motifs
- Dot Blot
- LacZ staining of cells
- NTA chip creation
- NTA surface preparation
- User:Leprevost/Purification of His-tagged proteins/Denaturing
- Generating antibodies
- Cell and tissue lysis hub
- Bioanalyzer
- Refolding Proteins
- Isolate leydig cells from testes
- Lactobacillus planarum miniprep
- Antibodies
- TE antigen retrieval and immunostaining
- Protein Purification - Salting Out
- Feruloyl Esterase Protocols
- Wwwblast
- Beta-galactosidase Screen
- Beta-glucuronidase protocols
- User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/05/23
- SDS-PAGE sample buffer (Morris formulation)
- Endy:E. coli Western Blot
- Sauer:Expression/purification of 35S-Met proteins
- Silver: Lysate for Western
- Silver: Coomassie Stain
- Silver: Destain
- Matsudaira:hsCen2 purification
- Keating:Experimental Protocols:HPLC
- Knight:Purification of His-tagged proteins/Denaturing
- Knight:NuPAGE electrophoresis
- Knight:Electrophoretic mobility shift assay
- Knight:Purification of His-tagged proteins
- Knight:Purification of His-tagged proteins/Native
- Knight:Protein solubility
- Knight:Purification of His-tagged proteins/Denaturing with refolding
- Knight:Centrifuge desalting
- Knight:Centrifuge desalting/Zeba columns
- Knight:NuPAGE electrophoresis/Fast staining
- Knight:NuPAGE electrophoresis/Slow staining
- Knight:NuPAGE electrophoresis/Hybrid staining
- Sauer:Lysing E. coli with Lysozymes
- Knight:Purification of MBP-tagged proteins
- Knight:NuPAGE electrophoresis/Gel drying
- Etchevers:ChIP francais
- Alm:Agarose gel electrophoresis
- Wikiomics:Percentage identity
- Wikiomics:Protein function prediction
- Wikiomics:Protein volume
- Myers:EGFR IP
- Jacobs:Protocol BCA Total Protein
- ISISBio:Protocols/p11 resin preparation
- Jacobs:Protocol Real-Time PCR
- Myers:ProteinComplexPurification
- ISISBio:Protocols/Sortase mediated ligation
- ISISBio:Protocols/Sortase mediated ligation/Small molecule ligation
- ISISBio:Protocols/Sortase mediated ligation/Solid support ligation
- ISISBio:Protocols/Sortase mediated ligation/Protein DNA ligation
- Jacobs:Protocol Subculturing 3T3 Fibroblasts
- Jacobs:Protocol RNA Isolation Using Tri Reagent
- Myers:Proteomics SOP
- Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein
- Jacobs:Protocol 10 bp DNA Ladder
- Jacobs:Protocol Total Protein Isolation using RIPA buffer
- Jacobs:Protocol Total Protein Isolation using RIPA buffer 2
- Jacobs:Protocol RNA Sample Loading Buffer
- Jacobs:Protocol Total RNA Isolation using Tri Reagent
- ISISBio:Protocols/Purification/Tus
- Griffin: Ultimate Immunoprecipitation Guide
- Griffin:Nuclear Extract Preparation
- Griffin:Western Blot Optimization
- Griffin:Immunohistochemistry Paraffin
- Griffin:Membrane Stripping and Reprobing
- Griffin:Antibody Elution Buffers
- Griffin:In Vitro Kinase Assay
- Griffin:Antibody Related Solutions & Recipes
- Griffin:Isolate Leukocytes from whole blood
- Fong:Bradford protein assay
- Prbbbb:small scale expression v1
- Prbbbb:Coomassi Ethanol stain
- Prbbbb:inclusion body solubilization v1
- Prbbbb:in vitro FRET FRB FKBP v1
- Jacobs: Protocol Immunostaining for TRPV4 and primary cilia
Materials
- MRFP1
- YFP
- CFP
- GFP
- Emerald
- Cell and tissue lysis hub
- Antibodies
- 3D printing models of biomolecules
- 3D printing ABS with PLA support
Resources
See Web resources#Protein and Web resources#Protein 2 and Web resources#Protein 3
