Protein blot (Western) hub: Difference between revisions
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m (Western blot (immunoblot) hub moved to Protein blot (Western) hub: Although widespread the compass puns of blotting methods are getting a bit stale (Northern, Southern, Far Western,..) and they are confusing to the newcomer.) |
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[[Image:Hub icon.png|right]] | [[Image:Hub icon.png|right]] | ||
The ''' | The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting. | ||
==General Western blot protocol== | ==General Western blot protocol== |
Revision as of 06:52, 16 April 2009
The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
General Western blot protocol
Western blot protocols by individual labs
- Endy:Tris-Tricine Acrylamide Gels
- Endy:E. coli Western Blot
- Endy: General Western Blot
- Odom:Western Blot
- Sauer:Western blot
- Ketner: Tissue culture viral lysate Western Blot
- Tissue preparation for western blot