Protein blot (Western) hub: Difference between revisions
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The ''' | The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting. | ||
==General | ==General protein blot protocol== | ||
* [[Western | * [[Protein blot (Western)]] | ||
==Western blot protocols by individual labs== | ==Western blot protocols by individual labs== | ||
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==See also== | ==See also== | ||
* [[Griffin:Membrane Stripping and Reprobing]] | * [[Griffin:Membrane Stripping and Reprobing]] | ||
* [[Griffin:Western Blot Optimization]] | |||
==External links== | ==External links== | ||
* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia] | * [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia] | ||
* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online] | * [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online] | ||
* [[Image:3stars.png]] [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ] | |||
[[Category:Protocol]] | [[Category:Protocol]] | ||
[[Category:In vitro]] | [[Category:In vitro]] | ||
[[Category:Protein]] | [[Category:Protein]] | ||
Latest revision as of 02:17, 27 June 2012
The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
General protein blot protocol
Western blot protocols by individual labs
- Endy:Tris-Tricine Acrylamide Gels
- Endy:E. coli Western Blot
- Endy: General Western Blot
- Odom:Western Blot
- Sauer:Western blot
- Ketner: Tissue culture viral lysate Western Blot
- Tissue preparation for western blot
