Protein blot (Western) hub - Revision history

Lucas Schirmer: /* See also */

2012-06-27T09:17:32Z

Jakob Suckale: /* General Western blot protocol */

2009-04-16T13:58:47Z

General Western blot protocol

←Older revision		Revision as of 13:58, 16 April 2009
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	The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics\|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.		The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics\|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.

-	==General ~~Western~~ blot protocol==	+	==General protein blot protocol==
-	* [[~~Western Blot~~ (~~immunoblot~~)]]	+	* [[Protein blot (Western)]]

	==Western blot protocols by individual labs==		==Western blot protocols by individual labs==

Jakob Suckale: lead section

2009-04-16T13:52:59Z

lead section

←Older revision		Revision as of 13:52, 16 April 2009
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	[[Image:Hub icon.png\|right]]		[[Image:Hub icon.png\|right]]

-	The '''~~Western~~ blot''', also ~~known~~ as ~~the immunoblot or the protein~~ blot, is one of the most tried and true experiments for [[Griffin:Antibody_Basics\|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.	+	The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics\|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.

	==General Western blot protocol==		==General Western blot protocol==

Jakob Suckale: Western blot (immunoblot) hub moved to Protein blot (Western) hub: Although widespread the compass puns of blotting methods are getting a bit stale (Northern, Southern, Far Western,..) and they are confusing to the newcomer.

2009-04-16T13:51:34Z

Western blot (immunoblot) hub moved to Protein blot (Western) hub: Although widespread the compass puns of blotting methods are getting a bit stale (Northern, Southern, Far Western,..) and they are confusing to the newcomer.

←Older revision

Revision as of 13:51, 16 April 2009

Jakob Suckale: /* External links */

2009-02-24T11:05:55Z

External links

←Older revision		Revision as of 11:05, 24 February 2009
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	* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]		* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]
	* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]		* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]
-	* [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ]	+	* [[Image:3stars.png]] [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ]

	[[Category:Protocol]]		[[Category:Protocol]]
	[[Category:In vitro]]		[[Category:In vitro]]
	[[Category:Protein]]		[[Category:Protein]]

Jakob Suckale: /* External links */

2009-02-24T11:05:17Z

External links

←Older revision		Revision as of 11:05, 24 February 2009
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	* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]		* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]
	* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]		* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]
		+	* [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ]

	[[Category:Protocol]]		[[Category:Protocol]]
	[[Category:In vitro]]		[[Category:In vitro]]
	[[Category:Protein]]		[[Category:Protein]]

Jakob Suckale: lead

2008-10-15T13:25:34Z

lead

←Older revision		Revision as of 13:25, 15 October 2008
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	[[Image:Hub icon.png\|right]]		[[Image:Hub icon.png\|right]]

-	The Western blot is one of the most tried and true experiments for [~~http://openwetware.org/wiki/~~Griffin:Antibody_Basics antibody]-dependent determination of protein expression/presence in a sample.	+	The '''Western blot''', also known as the immunoblot or the protein blot, is one of the most tried and true experiments for [[Griffin:Antibody_Basics\|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.

	==General Western blot protocol==		==General Western blot protocol==

Jakob Suckale: exchanged short intro from Western Blot (immunoblot) with long one from Western blot (immunoblot) hub

2008-10-15T13:21:07Z

exchanged short intro from Western Blot (immunoblot) with long one from Western blot (immunoblot) hub

←Older revision		Revision as of 13:21, 15 October 2008
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	[[Image:Hub icon.png\|right]]		[[Image:Hub icon.png\|right]]

-	~~==Overview==~~	+	The Western blot is one of the most tried and true experiments for [http://openwetware.org/wiki/Griffin:Antibody_Basics antibody]-dependent determination of protein expression/presence in a sample.
-	A Western ~~Blot allows~~ for ~~the semiquantitative~~ determination of protein expression.	+
-	Crude cell lysates are loaded into a polyacrylamide gel containing a denaturing agent which give all the proteins a net negative charge. A current can then be passed through the gel and the proteins will migrate through the gel, with the largest proteins traveling the slowest, resulting in a lane where proteins become separated on the basis of their weight. The proteins can then be transferred from the gel onto a membrane (often nitrocellulose or PVDF), which can then by incubated with an antibody directed against the protein of interest. By using a detector conjugated to an antibody one can now detect specifically the protein of interest.	+

	==General Western blot protocol==		==General Western blot protocol==

Jakob Suckale: /* See also */

2008-10-15T13:18:36Z

Jakob Suckale: see also

2008-10-15T13:18:19Z

←Older revision		Revision as of 09:17, 27 June 2012
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	==See also==		==See also==
	* [[Griffin:Membrane Stripping and Reprobing]]		* [[Griffin:Membrane Stripping and Reprobing]]
		+	* [[Griffin:Western Blot Optimization]]

	==External links==		==External links==

←Older revision		Revision as of 13:18, 15 October 2008
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	==See also==		==See also==
-	* [[Griffin:~~Membrane_Stripping_and_Reprobing~~ Membrane Stripping and Reprobing]]	+	* [[Griffin:Membrane Stripping and Reprobing]]

	==External links==		==External links==

←Older revision		Revision as of 13:18, 15 October 2008
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	*[[Ketner: Tissue culture viral lysate Western Blot]]		*[[Ketner: Tissue culture viral lysate Western Blot]]
	*[[Western Blot/Tissue Preparation\|Tissue preparation for western blot]]		*[[Western Blot/Tissue Preparation\|Tissue preparation for western blot]]
		+
		+	==See also==
		+	* [[Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]]

	==External links==		==External links==