|
|
Line 19: |
Line 19: |
|
| |
|
| |width="267px" class="green3"| | | |width="267px" class="green3"| |
| [[Category:Protocol]]
| |
|
| |
| Protocol for passing cells on fibronectin-coated dishes.
| |
|
| |
| 1. Prepare fibronectin-coated dishes.
| |
| Put 150ul fibronectin solution (20-40 ug/ml in water) at the center of glass-bottom dish, then overnight in CO2 incubator or 2 hours without cover in hood at RT.
| |
|
| |
| 2. Detach cells (HeLa, MEF) with 4 mM EDTA(in PBS) in CO2 incubator for 15 min, then slightly blow down the cells from the bottom of culture dish. The other steps are similar as normal pass.
| |
|
| |
| 3. Usually before imaging, incubate cells on fibronectin-coated dishes in CO2 incubator for 1-6 h.
| |
|
| |
|
| ==''In vivo''== | | ==''In vivo''== |