Protocols

In vivo Escherichia coli beta-Galactosidase Assay (A better Miller) Bacterial cell culture Preparing chemically competent cells Transforming chemically competent cells Electrocompetent cells Electroporation Miniprep Colony PCR Pulse-chase protein production Pour LB plates S_N Chemostat Protocol P1vir phage transduction Protein expression resource Mesoplasma florum Preparing culture media T7 Simple lysate protocol from plaque or lysate Cesium Chloride Purification of T7 Yeast High Efficiency Transformation Yeast Colony PCR Yeast DNA Prep Cell Cycle Arrest Fixing cells B-galactosidase assay Time-Lapse Microscopy Simple Yeast Lysate (for Western etc.) Assaying mating Pour YPD plates Dropout plates for yeast Layered plates

In vitro Nucleic acids Quantification of nucleic acids Phenol/chloroform extraction Nucleic acid precipitation Denaturing acrylamide gel purification of nucleic acids DNA Agarose gel electrophoresis Annealing Primers Designing primers DNA Ligation Library Generation Miniprep PCR Phosphatase treatment of linearized vector PNK Treatment of DNA Ends Purification of DNA Reconstituting primers Restriction digest 'Round-the-horn site-directed mutagenesis Sequencing DNA Site-directed mutagenesis Templiphi Church/Mitra Lab Polony Protocols RNA RNA Extraction Northern Blot, 32P End-Labeled Probes Probe Prep, 32P End-Labeled Probes RNase Protection Assay In vitro transcription with T7 RNA polymerase RNA Half-life Protein Acrylamide gels and SDS-PAGE Coomassie Stain Coomassie Destain Silver staining Western Blot HPLC

Other Flow Cytometry Flow Calibration Microfluidics Design a Microfluidic Chip Order a Microfluidic Chip from the Foundry Operate Microfluidic Chemostat Microscopy Agarose Pads for Microscopy Quantitative Microscopy Time-Lapse Microscopy Plate reader Perkin Elmer Victor3 plate reader usage protocols General resources Protocol-online- Contains a lot of useful protocols and a popular discussion section.