Protocols

In vivo Escherichia coli Bacterial cell culture Bacterial Transformation Preparing chemically competent cells Transforming chemically competent cells Preparing electrocompetent cells Transforming electrocompetent cells beta-Galactosidase Assay (A better Miller) Colony PCR Miniprep P1vir phage transduction Pour LB plates Protein expression resource Pulse-chase protein production S_N Chemostat Protocol Mesoplasma florum Preparing culture media T7 Cesium Chloride Purification of T7 Simple lysate protocol from plaque or lysate Yeast Assaying mating B-galactosidase assay Cell Cycle Arrest Dropout plates for yeast Fixing cells High Efficiency Transformation Layered plates Pour YPD plates Simple Yeast Lysate (for Western etc.) Time-Lapse Microscopy Yeast Colony PCR Yeast DNA Prep

In vitro Nucleic acids Denaturing acrylamide gel purification of nucleic acids Nucleic acid precipitation Phenol/chloroform extraction Quantification of nucleic acids DNA Agarose gel electrophoresis Annealing Primers Designing primers DNA Ligation Library Generation Miniprep PCR Phosphatase treatment of linearized vector Polony Protocols (Church/Mitra Lab) PNK Treatment of DNA Ends Purification of DNA Reconstituting primers Restriction digest 'Round-the-horn site-directed mutagenesis Sequencing DNA Site-directed mutagenesis Templiphi RNA In vitro transcription with T7 RNA polymerase Northern Blot, 32P End-Labeled Probes Probe Prep, 32P End-Labeled Probes RNA Extraction RNA Half-life RNase Protection Assay Protein Acrylamide gels and SDS-PAGE Coomassie Destain Coomassie Stain HPLC Silver staining Western Blot

Other Flow Cytometry Flow Calibration Microfluidics Design a Microfluidic Chip Order a Microfluidic Chip from the Foundry Operate Microfluidic Chemostat Microscopy Agarose Pads for Microscopy Quantitative Microscopy Time-Lapse Microscopy Plate reader Perkin Elmer Victor3 -Plate reader usage protocols General resources Protocol-online- Contains a lot of useful protocols and a popular discussion section.