Protocols: Difference between revisions

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==Protocols in Development==
==Protocols in Development==


'''''Please Contribute!'''''<BR>
'''''Please Contribute!'''''
* [[Pulse-chase protein production]]
* [[Pulse-chase protein production]]
* [[Fixing cells]]  
* [[Fixing cells]]  
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{{size|2|'''''Escherichia coli'''''}}__HIDER__
{{size|2|'''''Escherichia coli'''''}}__HIDER__
<hide>
<hide>
&bull; [[Bacterial cell culture|<font style="color:#000000">Bacterial Cell culture</font>]] <BR>
* [[Bacterial cell culture]]  
&bull; [[Bacterial Transformation |<font style="color:#000000">Bacterial Transformation</font>]] <BR>
* [[Bacterial Transformation ]]  
&nbsp;&nbsp;&bull; [[Preparing chemically competent cells|<font style="color:#000000" size=1>Preparing chemically competent cells (TSS)</font>]] <BR>
** [[Preparing chemically competent cells]]  
&nbsp;&nbsp;&bull; [[Transforming chemically competent cells|<font style="color:#000000" size=1>Transforming chemically competent cells (TSS)</font>]] <BR>
** [[Transforming chemically competent cells]]  
&nbsp;&nbsp;&bull; [[Electrocompetent cells|<font style="color:#000000" size=1>Preparing electrocompetent cells</font>]] <BR>
** [[Electrocompetent cells]]  
&nbsp;&nbsp;&bull; [[Electroporation|<font style="color:#000000" size=1>Transforming electrocompetent cells</font>]] <BR>
** [[Electroporation]]  
&bull; [[beta-Galactosidase Assay (A better Miller)|<font style="color:#000000">&beta;-Galactosidase Assay</font>]] <font size=1>(A better Miller)</font> <BR>
* [[beta-Galactosidase Assay (A better Miller)]]
&bull; [[Colony PCR|<font style="color:#000000">Colony PCR</font>]] <BR>
* [[Colony PCR]]  
&bull; [[Making a long term stock of bacteria|<font style="color:#000000">Making long-term stock bacteria</font>]] <BR>
* [[Making a long term stock of bacteria]]  
&bull; [[Miniprep|<font style="color:#000000">Miniprep</font>]] <BR>
* [[Miniprep]]  
&bull; [[P1vir phage transduction|<font style="color:#000000">P1vir phage transduction</font>]] <BR>
* [[P1vir phage transduction]]  
&bull; [[Endy:Pouring plates|<font style="color:#000000">Pour LB plates</font>]] <BR>
* [[Endy:Pouring plates]]  
&bull; [http://www.cf.ac.uk/biosi/staff/ehrmann/tools/factors.htm <font style="color:#000000">Protein expression resource</font>] <BR>
* [http://www.cf.ac.uk/biosi/staff/ehrmann/tools/factors.htm Protein expression resource]  
&bull; [[S_N Chemostat Protocol|<font style="color:#000000">S N Chemostat Protocol</font>]] <BR>
* [[S_N Chemostat Protocol]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''''Mesoplasma florum'''''}}__HIDER__
{{size|2|'''''Mesoplasma florum'''''}}__HIDER__
<hide>
<hide>
&bull; [[Mesoplama florum:Culture media|<font style="color:#000000">Preparing culture media</font>]] <BR>
* [[Mesoplama florum:Culture media|Culture media]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''T7'''}}__HIDER__
{{size|2|'''T7'''}}__HIDER__
<hide>
<hide>
&bull; [[Cesium Chloride Purification of T7|<font style="color:#000000">Purification T7: Cesium Chloride</font>]] <BR>
* [[Cesium Chloride Purification of T7]]  
&bull; [[Studier Lysate Prep|<font style="color:#000000">Simple lysate protocol from plaque/lysate</font>]] <BR>
* [[Studier Lysate Prep]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''Yeast'''}}__HIDER__
{{size|2|'''Yeast'''}}__HIDER__
<hide>
<hide>
&bull; [[Assaying mating|<font style="color:#000000">Assaying mating</font>]] <BR>
* [[Assaying mating]]  
&bull; [[Cell Cycle Arrest|<font style="color:#000000">Cell Cycle Arrest</font>]] <BR>
* [[Cell Cycle Arrest]]  
&bull; [[Dropout plates for yeast|<font style="color:#000000">Dropout plates</font>]] <BR>
* [[Dropout plates for yeast]]  
&bull; [[High Efficiency Transformation|<font style="color:#000000">High Efficiency Transformation</font>]] <BR>
* [[High Efficiency Transformation]]  
&bull; [[Layered plates|<font style="color:#000000">Layering plates</font>]] <BR>
* [[Layered plates]]  
&bull; [[Pour YPD plates|<font style="color:#000000">Pour YPD plates</font>]] <BR>
* [[Pour YPD plates]]  
&bull; [[Silver: Lysate for Western|<font style="color:#000000">Simple Yeast Lysate</font>]] <font size=1>(for Western etc.)</font> <BR>
* [[Silver: Lysate for Western]] <font size=1>(for Western etc.)</font>  
&bull; [[Silver: Time-Lapse Microscopy|<font style="color:#000000">Time-Lapse Microscopy</font>]] <BR>
* [[Silver: Time-Lapse Microscopy]]  
&bull; [[Yeast Colony PCR|<font style="color:#000000">Yeast Colony PCR</font>]] <BR>
* [[Yeast Colony PCR]]  
&bull; [[Yeast DNA Prep|<font style="color:#000000">Yeast DNA Prep</font>]] <BR>
* [[Yeast DNA Prep]]  
</hide>
</hide>
</showhide>
</showhide>
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</showhide>
</showhide>
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<!------------------------------------------------------------------------------->
<!-----------------------------------Blank temp---------------------------------->
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<!------------------------------------------------------------------------------->
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<!-----------------------------------In vitro----------------------------------->
<!-----------------------------------In vitro----------------------------------->
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{{size|2|'''Nucleic acids'''}}__HIDER__
{{size|2|'''Nucleic acids'''}}__HIDER__
<hide>
<hide>
&bull; [[Nucleic acid precipitation|<font style="color:#000000">Nucleic acid precipitation</font>]] <BR>
* [[Nucleic acid precipitation]]  
&bull; [[Phenol/chloroform extraction|<font style="color:#000000">Phenol/chloroform extraction</font>]] <BR>
* [[Phenol/chloroform extraction]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''DNA'''}}__HIDER__
{{size|2|'''DNA'''}}__HIDER__
<hide>
<hide>
&bull; [[Agarose gel electrophoresis|<font style="color:#000000">Agarose gel electrophoresis</font>]] <BR>
* [[Agarose gel electrophoresis]]  
&bull; [[Annealing Primers|<font style="color:#000000">Annealing Primers</font>]] <BR>
* [[Annealing Primers]]  
&bull; [[Designing primers|<font style="color:#000000">Designing Primers</font>]] <BR>
* [[Designing primers]]  
&bull; [[DNA Ligation|<font style="color:#000000">DNA Ligation</font>]] <BR>
* [[DNA Ligation]]  
&bull; [[Double stranding oligo libraries|<font style="color:#000000">Double stranding oligo libraries</font>]] <BR>
* [[Double stranding oligo libraries]]  
&bull; [[Library Generation|<font style="color:#000000">Library Generating</font>]] <BR>
* [[Library Generation]]  
&bull; [[Miniprep|<font style="color:#000000">Miniprep</font>]] <BR>
* [[Miniprep]]  
&bull; [[PCR|<font style="color:#000000">PCR</font>]] <BR>
* [[PCR]]  
&bull; [[Phosphatase treatment of linearized vector|<font style="color:#000000">Phosphate treatment linearized vector</font>]] <BR>
* [[Phosphatase treatment of linearized vector]]  
&bull; [http://arep.med.harvard.edu/polony/polony_protocols <font style="color:#000000">Polony Protocols</font>]<font size=1>(Church/Mitra Lab)</font> <BR>
* [http://arep.med.harvard.edu/polony/polony_protocols Polony Protocols]
&bull; [[PNK Treatment of DNA Ends|<font style="color:#000000">PNK Treatment of DNA ends</font>]] <BR>
* [[PNK Treatment of DNA Ends]]  
&bull; [[Purification of DNA|<font style="color:#000000">Purification of DNA</font>]] <BR>
* [[Purification of DNA]]  
&bull; [[Reconstituting primers|<font style="color:#000000">Reconstituting Primers</font>]] <BR>
* [[Reconstituting primers]]  
&bull; [[Restriction digest|<font style="color:#000000">Restriction Digest</font>]] <BR>
* [[Restriction digest]]  
&bull; [['Round-the-horn site-directed mutagenesis|<font style="color:#000000">'Round-the-horn site-directed mutagenesis</font>]] <BR>
* [['Round-the-horn site-directed mutagenesis]]  
&bull; [[Sequencing DNA|<font style="color:#000000">Sequencing DNA</font>]] <BR>
* [[Sequencing DNA]]  
&bull; [[Site-directed mutagenesis|<font style="color:#000000">Site-directed Mutagenesis</font>]] <BR>
* [[Site-directed mutagenesis]]  
&bull; [[Templiphi|<font style="color:#000000">Templiphi</font>]] <BR>
* [[Templiphi]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''RNA'''}}__HIDER__
{{size|2|'''RNA'''}}__HIDER__
<hide>
<hide>
&bull; [[In vitro transcription with T7 RNA polymerase|<font style="color:#000000">In vitro transcription w/ T7 RNA polymerase</font>]] <BR>
* [[In vitro transcription with T7 RNA polymerase]]  
&bull; [[Northern Blot, 32P End-Labeled Probes |<font style="color:#000000">Northern Blot, <sup>32</sup>P End-Labeled Probes</font>]] <BR>
* [[Northern Blot, 32P End-Labeled Probes ]]  
&bull; [[Probe Prep, 32P End-Labeled Probes|<font style="color:#000000">Probe Prep, <sup>32</sup>P End-Labeled Probes</font>]] <BR>
* [[Probe Prep, 32P End-Labeled Probes]]  
&bull; [[RNA Extraction|<font style="color:#000000">RNA Extraction</font>]] <BR>
* [[RNA Extraction]]  
&bull; [[RNase Protection Assay|<font style="color:#000000">RNAse Protection Assay</font>]] <BR>
* [[RNase Protection Assay]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''Protein'''}}__HIDER__
{{size|2|'''Protein'''}}__HIDER__
<hide>
<hide>
&bull; [[Acrylamide Gels|<font style="color:#000000">Acrylamide gels and SDS-PAGE</font>]] <BR>
* [[Acrylamide Gels]]  
&bull; [[Silver: Destain|<font style="color:#000000">Coomassie Destain</font>]] <BR>
* [[Silver: Destain]]  
&bull; [[Silver: Coomassie Stain|<font style="color:#000000">Coomassie Stain</font>]] <BR>
* [[Silver: Coomassie Stain]]  
&bull; [[Keating:Experimental Protocols:HPLC|<font style="color:#000000">HPLC</font>]] <BR>
* [[Keating:Experimental Protocols:HPLC]]  
&bull; [[Silver staining|<font style="color:#000000">Silver staining</font>]] <BR>
* [[Silver staining]]  
&bull; [[Western Blot|<font style="color:#000000">Western Blot</font>]] <BR>
* [[Western Blot]]  
&bull; [[Purification of His-tagged proteins|<font style="color:#000000">Purification His-tagged proteins</font>]] <BR>
* [[Purification of His-tagged proteins]]  
&bull; [[Free Sulfhydryl Determination|<font style="color:#000000">Free Sulfhydryl Determination</font>]]
* [[Free Sulfhydryl Determination]]
</hide>
</hide>
</showhide>
</showhide>
</div>


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|-


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<!------------------------------------------------------------------------------->
<!------------------------------------------------------------------------------->
<!-----------------------------------In silico------------------------------------>
<!-----------------------------------In silico------------------------------------>
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{{size|2|'''Sequence Analysis'''}}__HIDER__
{{size|2|'''Sequence Analysis'''}}__HIDER__
<hide>
<hide>
&bull; [[BLAST|<font style="color:#000000">BLAST</font>]] <BR>
* [[BLAST]]  
&bull; [[CLUSTALW|<font style="color:#000000">CLUSTALW</font>]] <BR>
* [[CLUSTALW]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''Structure'''}}__HIDER__
{{size|2|'''Structure'''}}__HIDER__
<hide>
<hide>
&bull; [[THREADER|<font style="color:#000000">THREADER</font>]] <BR>
* [[THREADER]]  
&bull; [[Homology Modelling|<font style="color:#000000">Homology Modeling</font>]] <BR>
* [[Homology Modelling]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''Pathway Modelling'''}}__HIDER__
{{size|2|'''Pathway Modelling'''}}__HIDER__
<hide>
<hide>
&bull; [[FBA|<font style="color:#000000">Flux Balance Analysis</font>]] <BR>
* [[FBA]]  
&bull; [[MoMA|<font style="color:#000000">Minimisation of Metabolic Adjustment</font>]] <BR>
* [[MoMA]]  
</hide>
</hide>
</showhide>
</showhide>
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<!------------------------------------------------------------------------------->
<!------------------------------------------------------------------------------->
<!-----------------------------------OTHER--------------------------------------->
<!-----------------------------------OTHER--------------------------------------->
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{{size|2|'''Flow Cytometry'''}}__HIDER__
{{size|2|'''Flow Cytometry'''}}__HIDER__
<hide>
<hide>
&bull; [[Flow Calibration|<font style="color:#000000">Flow Calibration</font>]] <BR>
* [[Flow Calibration]]  
&bull; [[Endy:FACS protocols|<font style="color:#000000">Endy/Knight Labs FACS protocols</font>]] <BR>
* [[Endy:FACS protocols]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''Microfluidics'''}}__HIDER__
{{size|2|'''Microfluidics'''}}__HIDER__
<hide>
<hide>
&bull; [[Design a Microfluidic Chip|<font style="color:#000000">Design Microfluidic Chip</font>]] <BR>
* [[Design a Microfluidic Chip]]  
&bull; [[Order a Microfluidic Chip from the Foundry|<font style="color:#000000">Order Microfluidic Chip from Foundry</font>]] <BR>
* [[Order a Microfluidic Chip from the Foundry]]  
&bull; [[Operate Microfluidic Chemostat|<font style="color:#000000">Operate Microfluidic Chemostat</font>]] <BR>
* [[Operate Microfluidic Chemostat]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''Microscopy'''}}__HIDER__
{{size|2|'''Microscopy'''}}__HIDER__
<hide>
<hide>
&bull; [[Agarose Pads for Microscopy|<font style="color:#000000">Agarose Pads</font>]] <BR>
* [[Agarose Pads for Microscopy]]  
&bull; [[Quantitative Microscopy|<font style="color:#000000">Quantitative Microscopy</font>]] <BR>
* [[Quantitative Microscopy]]  
&bull; [[Silver: Time-Lapse Microscopy|<font style="color:#000000">Time-Lapse Microscopy</font>]] <BR>
* [[Silver: Time-Lapse Microscopy]]  
</hide>
</hide>
</showhide>
</showhide>
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{{size|2|'''Plate reader'''}}__HIDER__
{{size|2|'''Plate reader'''}}__HIDER__
<hide>
<hide>
&bull; [[Endy:Victor3 plate reader|<font style="color:#000000">Perkin Elmer Victor<sup>3</sup></font>]]<font size=1><BR>Plate reader usage protocols</font> <BR>
* [[Endy:Victor3 plate reader]]<font size=1>Plate reader usage protocols</font>  
</hide>
</hide>
</showhide>
</showhide>


{{size|2|'''General resources'''}}<BR>
{{size|2|'''General resources'''}}
&bull; [http://protocol-online.org <font style="color:#000000">Protocol-online</font>] <font size=1><BR>Contains a lot of useful protocols and a popular discussion section.</font> <BR>
* [http://protocol-online.org Protocol-online] <font size=1>Contains a lot of useful protocols and a popular discussion section.</font>  
 
 
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Revision as of 14:47, 30 March 2006

Protocols in Development

Please Contribute!

In vivo

<showhide> Escherichia coli__HIDER__ <hide>

</hide> </showhide>

<showhide> Mesoplasma florum__HIDER__ <hide>

</hide> </showhide>

<showhide> T7__HIDER__ <hide>

</hide> </showhide>

<showhide> Yeast__HIDER__ <hide>

</hide> </showhide>

<showhide> Mouse__HIDER__ <hide>

</hide> </showhide>

In vitro

<showhide> Nucleic acids__HIDER__ <hide>

</hide> </showhide>

<showhide> DNA__HIDER__ <hide>

</hide> </showhide>

<showhide> RNA__HIDER__ <hide>

</hide> </showhide>

<showhide> Protein__HIDER__ <hide>

</hide> </showhide>

In silico

<showhide> Sequence Analysis__HIDER__ <hide>

</hide> </showhide>

<showhide> Structure__HIDER__ <hide>

</hide> </showhide>

<showhide> Pathway Modelling__HIDER__ <hide>

</hide> </showhide>

Other

<showhide> Flow Cytometry__HIDER__ <hide>

</hide> </showhide>

<showhide> Microfluidics__HIDER__ <hide>

</hide> </showhide>

<showhide> Microscopy__HIDER__ <hide>

</hide> </showhide>

<showhide> Plate reader__HIDER__ <hide>

</hide> </showhide>

General resources

  • Protocol-online Contains a lot of useful protocols and a popular discussion section.

<showhide> __SHOWALL__ __HIDEALL__ </showhide>

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