Protocols: Difference between revisions
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Revision as of 16:34, 9 November 2005
In vivo
Escherichia coli
Pulse-chase protein production
Yeast
High Efficiency Transformation
Simple Yeast Lysate (for Western etc.)
T7
Simple lysate protocol from plaque or lysate
Cesium Chloride Purification of T7
Mesoplasma florum
In vitro
Nucleic acids
Quantification of nucleic acids
Denaturing acrylamide gel purification of nucleic acids
DNA
Phosphatase treatment of linearized vector
'Round-the-horn site-directed mutagenesis
RNA
Northern Blot, 32P End-Labeled Probes
Probe Prep, 32P End-Labeled Probes
In vitro transcription with T7 RNA polymerase
Protein
Microfluidics
Order a Microfluidic Chip from the Foundry
Operate Microfluidic Chemostat
Microscopy
Flow Cytometry
Miscellaneous
Counteracting evaporation from wells in a Victor3 plate reader
