Protocols

---

---

Protocols in development Please Contribute! Pulse-chase protein production Fixing cells Real-time RT-PCR β-galactosidase assay Denaturing acrylamide gel purification of nucleic acids Quantification of nucleic acids Bacterial species identification Yeast artificial chromosomes One step 'miniprep' method for the isolation of plasmid DNA Adding new protocols	In vivo <showhide> Drosophila__HIDER__ <hide> Drosophila CHiP on Chip </hide> </showhide> <showhide> Escherichia coli__HIDER__ <hide> Bacterial cell culture Bacterial Transformation Preparing chemically competent cells Transforming chemically competent cells Electrocompetent cells Electroporation Preparing chemically competent cells (Inoue) Transforming chemically competent cells (Inoue) TOP10 chemically competent cells beta-Galactosidase Assay (A better Miller) Colony PCR Making a long term stock of bacteria Miniprep P1vir phage transduction Recombineering Endy:Pouring plates Protein expression resource S_N Chemostat Protocol </hide> </showhide> <showhide> Mesoplasma florum__HIDER__ <hide> Culture media </hide> </showhide> <showhide> Mouse__HIDER__ <hide> Mouse keratinocyte cultures </hide> </showhide> <showhide> T7__HIDER__ <hide> Cesium Chloride Purification of T7 Studier Lysate Prep </hide> </showhide> <showhide> Yeast__HIDER__ <hide> Assaying mating Cell Cycle Arrest Dropout plates for yeast High Efficiency Transformation Layered plates Pour YPD plates Silver: Lysate for Western (for Western etc.) Silver: Time-Lapse Microscopy Yeast Colony PCR Yeast DNA Prep </hide> </showhide>	In vitro <showhide> Nucleic acids__HIDER__ <hide> Nucleic acid precipitation Phenol/chloroform extraction </hide> </showhide> <showhide> DNA__HIDER__ <hide> Agarose gel electrophoresis Annealing Primers Designing primers DNA Ligation DNA Synthesis from Oligos Double stranding oligo libraries Library Generation Miniprep PCR PCR Overlap Extension Phosphatase treatment of linearized vector Polony Protocols PNK Treatment of DNA Ends Purification of DNA Reconstituting primers Restriction digest 'Round-the-horn site-directed mutagenesis Sequencing DNA Site-directed mutagenesis Templiphi </hide> </showhide> <showhide> RNA__HIDER__ <hide> In vitro transcription with T7 RNA polymerase Northern Blot, 32P End-Labeled Probes Probe Prep, 32P End-Labeled Probes RNA Extraction RNase Protection Assay RNA Half-life </hide> </showhide> <showhide> Protein__HIDER__ <hide> Acrylamide Gels Silver: Destain Silver: Coomassie Stain Keating:Experimental Protocols:HPLC Silver staining Protein Preps: Yeast Denaturing Protein Extraction Native Protein Extraction Western Blots Pouring Tris-Tricine Acrylamide Gels E. coli Western Blot General Western Blot Purification of His-tagged proteins Free Sulfhydryl Determination Expression and purification of 35S-labeled proteins </hide> </showhide>
	In silico <showhide> Sequence Analysis__HIDER__ <hide> BLAST CLUSTALW </hide> </showhide> <showhide> Structure__HIDER__ <hide> THREADER Homology Modelling Coot and WinCoot for X-ray Crystallography Model Building </hide> </showhide> <showhide> Pathway Modelling__HIDER__ <hide> FBA MoMA </hide> </showhide>	Other <showhide> Flow Cytometry__HIDER__ <hide> Flow Calibration Endy:FACS protocols </hide> </showhide> <showhide> Microfluidics__HIDER__ <hide> Design a Microfluidic Chip Order a Microfluidic Chip from the Foundry Operate Microfluidic Chemostat </hide> </showhide> <showhide> Microscopy__HIDER__ <hide> Agarose Pads for Microscopy Quantitative Microscopy Silver: Time-Lapse Microscopy </hide> </showhide> <showhide> Plate reader__HIDER__ <hide> Endy:Victor3 plate readerPlate reader usage protocols </hide> </showhide> <showhide> General resources__HIDER__ <hide> Current Protocols in Molecular Biology Downloadable pdfs. Molecular Cloning Protocol-online Useful protocols and a popular discussion section. iProtocol Abridged Current Protocols Invitrogen protocols. Not amenable to printing. </hide> </showhide>

<showhide> __SHOWALL__ __HIDEALL__ </showhide>