Protocols
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In vivo
Escherichia coli
beta-Galactosidase Assay (A better Miller)
Bacterial cell culture
Preparing chemically competent cells
Transforming chemically competent cells
Electrocompetent cells
Electroporation
Miniprep
Colony PCR
Pulse-chase protein production
Pour LB plates
S_N Chemostat Protocol
P1vir phage transduction
Protein expression resource
Mesoplasma florum
Preparing culture media
T7
Simple lysate protocol from plaque or lysate
Cesium Chloride Purification of T7
Yeast
High Efficiency Transformation
Yeast Colony PCR
Yeast DNA Prep
Cell Cycle Arrest
Fixing cells
B-galactosidase assay
Time-Lapse Microscopy
Simple Yeast Lysate
(for Western etc.)
Assaying mating
Pour YPD plates
Dropout plates for yeast
Layered plates
In vitro
Nucleic acids
Quantification of nucleic acids
Phenol/chloroform extraction
Nucleic acid precipitation
Denaturing acrylamide gel purification of nucleic acids
DNA
Agarose gel electrophoresis
Annealing Primers
Designing primers
DNA Ligation
Library Generation
Miniprep
PCR
Phosphatase treatment of linearized vector
PNK Treatment of DNA Ends
Purification of DNA
Reconstituting primers
Restriction digest
'Round-the-horn site-directed mutagenesis
Sequencing DNA
Site-directed mutagenesis
Templiphi
Church/Mitra Lab Polony Protocols
RNA
RNA Extraction
Northern Blot,
32
P End-Labeled Probes
Probe Prep,
32
P End-Labeled Probes
RNase Protection Assay
In vitro transcription with T7 RNA polymerase
RNA Half-life
Protein
Acrylamide gels and SDS-PAGE
Coomassie Stain
Coomassie Destain
Silver staining
Western Blot
HPLC
Other
Flow Cytometry
Flow Calibration
Microfluidics
Design a Microfluidic Chip
Order a Microfluidic Chip from the Foundry
Operate Microfluidic Chemostat
Microscopy
Agarose Pads for Microscopy
Quantitative Microscopy
Time-Lapse Microscopy
Plate reader
Perkin Elmer Victor
3
plate reader usage protocols
General resources
Protocol-online
- Contains a lot of useful protocols and a popular discussion section.
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