Protocols
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Barry Canton
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In vivo
Escherichia coli
Bacterial cell culture
Bacterial Transformation
Preparing chemically competent cells (TSS)
Transforming chemically competent cells (TSS)
Preparing electrocompetent cells
Transforming electrocompetent cells
β-Galactosidase Assay (A better Miller)
Colony PCR
Miniprep
P1vir phage transduction
Pour LB plates
Protein expression resource
S_N Chemostat Protocol
Mesoplasma florum
Preparing culture media
T7
Cesium Chloride Purification of T7
Simple lysate protocol from plaque or lysate
Yeast
Assaying mating
Cell Cycle Arrest
Dropout plates for yeast
High Efficiency Transformation
Layered plates
Pour YPD plates
Simple Yeast Lysate
(for Western etc.)
Time-Lapse Microscopy
Yeast Colony PCR
Yeast DNA Prep
In vitro
Nucleic acids
Nucleic acid precipitation
Phenol/chloroform extraction
DNA
Agarose gel electrophoresis
Annealing Primers
Designing primers
DNA Ligation
Library Generation
Miniprep
PCR
Phosphatase treatment of linearized vector
Polony Protocols (Church/Mitra Lab)
PNK Treatment of DNA Ends
Purification of DNA
Reconstituting primers
Restriction digest
'Round-the-horn site-directed mutagenesis
Sequencing DNA
Site-directed mutagenesis
Templiphi
RNA
In vitro transcription with T7 RNA polymerase
Northern Blot,
32
P End-Labeled Probes
Probe Prep,
32
P End-Labeled Probes
RNA Extraction
RNase Protection Assay
Protein
Acrylamide gels and SDS-PAGE
Coomassie Destain
Coomassie Stain
HPLC
Silver staining
Western Blot
Other
Flow Cytometry
Flow Calibration
Microfluidics
Design a Microfluidic Chip
Order a Microfluidic Chip from the Foundry
Operate Microfluidic Chemostat
Microscopy
Agarose Pads for Microscopy
Quantitative Microscopy
Time-Lapse Microscopy
Plate reader
Perkin Elmer Victor
3
-Plate reader usage protocols
General resources
Protocol-online
- Contains a lot of useful protocols and a popular discussion section.
Protocols in Development
Please Contribute!
Pulse-chase protein production
Fixing cells
B-galactosidase assay
RNA Half-life
Denaturing acrylamide gel purification of nucleic acids
Quantification of nucleic acids
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