Protocols

In vivo <showhide> Escherichia coli__HIDER__ <hide> Bacterial cell culture Bacterial transformation Preparing chemically competent cells (TSS) Transforming chemically competent cells (TSS) Preparing electrocompetent cells Transforming electrocompetent cells β-Galactosidase Assay (A better Miller) Colony PCR Making a long term stock of bacteria Miniprep P1vir phage transduction Pour LB plates Protein expression resource S_N Chemostat Protocol </hide> </showhide> <showhide> Mesoplasma florum__HIDER__ <hide> Preparing culture media </hide> </showhide> <showhide> T7__HIDER__ <hide> Cesium Chloride Purification of T7 Simple lysate protocol from plaque or lysate </hide> </showhide> <showhide> Yeast__HIDER__ <hide> Assaying mating Cell Cycle Arrest Dropout plates for yeast High Efficiency Transformation Layered plates Pour YPD plates Simple Yeast Lysate (for Western etc.) Time-Lapse Microscopy Yeast Colony PCR Yeast DNA Prep </hide> </showhide>

In vitro <showhide> Nucleic acids__HIDER__ <hide> Nucleic acid precipitation Phenol/chloroform extraction </hide> </showhide> <showhide> DNA__HIDER__ <hide> Agarose gel electrophoresis Annealing Primers Designing primers DNA Ligation Library Generation Miniprep PCR Phosphatase treatment of linearized vector Polony Protocols (Church/Mitra Lab) PNK Treatment of DNA Ends Purification of DNA Reconstituting primers Restriction digest 'Round-the-horn site-directed mutagenesis Sequencing DNA Site-directed mutagenesis Templiphi </hide> </showhide> <showhide> RNA__HIDER__ <hide> In vitro transcription with T7 RNA polymerase Northern Blot, 32P End-Labeled Probes Probe Prep, 32P End-Labeled Probes RNA Extraction RNase Protection Assay </hide> </showhide> <showhide> Protein__HIDER__ <hide> Acrylamide gels and SDS-PAGE Coomassie Destain Coomassie Stain HPLC Silver staining Western Blot Purification of His-tagged proteins </hide> </showhide>

In silico <showhide> Sequence Analysis__HIDER__ <hide> BLAST CLUSTALW </hide> </showhide> <showhide> Structure__HIDER__ <hide> THREADER Homology Modelling </hide> </showhide> <showhide> Pathway Modelling__HIDER__ <hide> Flux Balance Analysis Minimisation of Metabolic Adjustment </hide> </showhide> Other <showhide> Flow Cytometry__HIDER__ <hide> Flow Calibration </hide> </showhide> <showhide> Microfluidics__HIDER__ <hide> Design a Microfluidic Chip Order a Microfluidic Chip from the Foundry Operate Microfluidic Chemostat </hide> </showhide> <showhide> Microscopy__HIDER__ <hide> Agarose Pads for Microscopy Quantitative Microscopy Time-Lapse Microscopy </hide> </showhide> <showhide> Plate reader__HIDER__ <hide> Perkin Elmer Victor3 -Plate reader usage protocols </hide> </showhide> General resources Protocol-online- Contains a lot of useful protocols and a popular discussion section. Protocols in Development Please Contribute! Pulse-chase protein production Fixing cells B-galactosidase assay RNA Half-life Denaturing acrylamide gel purification of nucleic acids Quantification of nucleic acids

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