Protocols
From OpenWetWare
In vivo
Escherichia coli
Pulse-chase protein production
Yeast
High Efficiency Transformation
T7
Simple lysate protocol from plaque or lysate
Cesium Chloride Purification of T7
Mesoplasma florum
In vitro
Nucleic acids
Quantification of nucleic acids
Denaturing acrylamide gel purification of nucleic acids
DNA
Phosphatase treatment of linearized vector
RNA
Northern Blot, 32P End-Labeled Probes
Probe Prep, 32P End-Labeled Probes
In vitro transcription with T7 RNA polymerase
Protein
Microfluidics
Order a Microfluidic Chip from the Foundry
Operate Microfluidic Chemostat
Microscopy
Miscellaneous
Counteracting Evaporation from 96 Well Plates in Long Time Courses on a Victor3 Plate Reader
