Protocols/Consensus template

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'''Click the view source tab and copy everything below this line.  Paste it into your new protocol page.  Then replace the text in this page with your own protocol.  Feel free to add or delete sections as appropriate.'''
'''Click the view source tab and copy everything below this line.  Paste it into your new protocol page.  Then replace the text in this page with your own protocol.  Feel free to add or delete sections as appropriate.'''
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<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL!  -->
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<!-- COPY EVERTYHING BELOW HERE TO START YOUR OWN PROTOCOL!  -->
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==General Information==
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==Author==
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Add authors here and their contact info e.g.<br>
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James Hadfield, CRUK Cambridge Research Institute, Robinson Way, Cambridge CB2 0RE. Tel: +44 (0)1223 404250; Fax: +44 (0)1223 404208; email: james.hadfield@cancer.org.uk.
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Replace this sentence with a brief description of the protocol and its goal. It might also be good to add an image to show the workflow and timescales for experiment planning.
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==Abstract==
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Replace this sentence with a brief description of the protocol and its goal.
==Materials==
==Materials==
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List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page.  Links to the suppliers' page on that material are also appropriate and encouraged.
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List everything necessary to perform the protocol here. Include all information about suppliers, ordering details, etc.  Links to the suppliers' page on that material are also appropriate and encouraged. Please be aware that users of this protocol may not be working in the same country as you.
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===Reagents===
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Biological resources e.g. cell lines, buffers (link to a method for making them), enzymes, chemicals, kits, etc.
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===Equipment===
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Any equipment used to perform the protocol (link to a method for using them).
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*reagent 1 [www.google.com spupplier page], Part Number, Pricing (perhaps we ca get an idea of how hard companies can be squeezed???)
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==Protocol==
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*supply 1 (i.e. tubes of a certain size? spreaders?)
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A step by step guide to the experimental procedure.
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*equipment 1
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==Protocol Procedure==
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===Step 1===
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Do step 1
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===Step 2===
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Do step 2
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Step 2 has some additional information that goes with it.  i.e. Keep at 4&deg;C.
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===Step 3===
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Do step 3
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Step 3 has multiple sub-steps within it.
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#Enumerate each of those...
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#As you need to...
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==Critical steps==
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Referenced from the main protocol, a more thorough explanation of particularly important steps in the protocol.
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==Troubleshooting==
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Referenced from the main protocol, an explanation of what can cause things to go wrong with the protocol.
==Notes==
==Notes==
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#Anyone should add info here and, at the very least, link to their reasoning.
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Referenced from the main protocol, any comments about the protocol should be made here; i.e. how it was developed. Any comments added should be signed (by adding <nowiki>~~~~</nowiki> in front) and explained. Links to FAQs/tips provided by other sources such as the manufacturer or other websites would be best made here.<br>
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#List troubleshooting tips here.
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Anecdotal observations that might be of use to others can also be posted here; e.g. 'my cells were still floating'.<br>
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#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
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It might also be good to add an image to show the workflow and timescales for experiment planning.
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#Anecdotal observations that might be of use to others can also be posted here.
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==Acknowledgments==
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Acnkowledge any help you had in development, testing, writing this protocol.
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Please sign your name to your note by adding <font face="courier"><nowiki>('''~~~~''')</nowiki></font> to the end of your tip.
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==References==
==References==
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'''Relevant papers and books'''
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See [[OpenWetWare:Biblio]] for information on how to reference within a wiki.
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<!-- If this protocol has papers or books associated with it, list those references here.  See the [[OpenWetWare:Biblio]] page for more information. -->
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<biblio>
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#Goldbeter-PNAS-1981 pmid=6947258
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</biblio>
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==Specific Protocols==
==Specific Protocols==
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Add links to other OWW protocols that have been used in making the consensus. Users should add their lab specific protocols to this list as they see fit.
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Add links to all the OWW protocols that have been used in making the consensus.
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==Contact==
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*Who has experience with this protocol?
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*Who wrote this protocol in the first place?
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Revision as of 11:38, 1 November 2006

This page is a template and should not be edited.
Click template&action=edit here, copy the source, and paste it into your page.



I am interested in posting a new template for consensus protocols on OpenWetWare? Here is a template to help you do so.

Click the view source tab and copy everything below this line. Paste it into your new protocol page. Then replace the text in this page with your own protocol. Feel free to add or delete sections as appropriate.

Contents

Author

Add authors here and their contact info e.g.
James Hadfield, CRUK Cambridge Research Institute, Robinson Way, Cambridge CB2 0RE. Tel: +44 (0)1223 404250; Fax: +44 (0)1223 404208; email: james.hadfield@cancer.org.uk.

Abstract

Replace this sentence with a brief description of the protocol and its goal.

Materials

List everything necessary to perform the protocol here. Include all information about suppliers, ordering details, etc. Links to the suppliers' page on that material are also appropriate and encouraged. Please be aware that users of this protocol may not be working in the same country as you.

Reagents

Biological resources e.g. cell lines, buffers (link to a method for making them), enzymes, chemicals, kits, etc.

Equipment

Any equipment used to perform the protocol (link to a method for using them).

Protocol

A step by step guide to the experimental procedure.

Critical steps

Referenced from the main protocol, a more thorough explanation of particularly important steps in the protocol.

Troubleshooting

Referenced from the main protocol, an explanation of what can cause things to go wrong with the protocol.

Notes

Referenced from the main protocol, any comments about the protocol should be made here; i.e. how it was developed. Any comments added should be signed (by adding ~~~~ in front) and explained. Links to FAQs/tips provided by other sources such as the manufacturer or other websites would be best made here.
Anecdotal observations that might be of use to others can also be posted here; e.g. 'my cells were still floating'.
It might also be good to add an image to show the workflow and timescales for experiment planning.

Acknowledgments

Acnkowledge any help you had in development, testing, writing this protocol.

References

See OpenWetWare:Biblio for information on how to reference within a wiki.

Specific Protocols

Add links to all the OWW protocols that have been used in making the consensus.

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