Protocols/Template: Difference between revisions

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{|style="width: 48em; background: #FF6677;"
| align="center"|'''This page is a template and should not be edited.'''<br><span style="font-size:90%">Click [http://openwetware.org/index.php?title={{PAGENAME}}&action=edit here], copy the source, and paste it into your page.</span>
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'''Interested in posting a protocol on OpenWetWare?  Here is a template to help you do so.''' 
'''Click the view source tab and copy everything below this line.  Paste it into your new protocol page.  Then replace the text in this page with your own protocol.  Feel free to add or delete sections as appropriate.'''
<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL!  -->
==Overview==
==Overview==


Use a mechanical hot press to create the base to which a microfluidic chip with channels can be bonded.
Replace this sentence with a brief description of the protocol and its goal.


==Materials==
==Materials==
List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.


*Mechanical Hot Press
*supply 1 (i.e. tubes of a certain size? spreaders?)
*Zeonex beads
*reagent 1
*2 Removable nickel plates
*X &mu;L reagent 2
**component A (reagent 2 is made up of multiple components)
**component B
*equipment 1
*equipment 2


==Procedure==
==Procedure==
#Set the temperature of platen 1 and 2 of the Hot Press to be around 330&deg;F.
#Step 1
#Center a circular arrangement of Zeonex beads on one of the nickel plates.
#Step 2
#Place the nickel plate onto the bottom Platen of the Hot Press, and carefully place the other nickel plate on top without disrupting the arrangement of Zeonex beads.  
#*Step 2 has some additional information that goes with it.  i.e. Keep at 4&deg;C.
#Pump the hydralic pressure handle until the platens touch and the pressure reading remains 0, and then wait 1 minute. This time allows the plastic to begin to warm and melt.
#Step 3
#Pump the hydralic handle until it reads 1000 psi.
##Step 3 has multiple sub-steps within it.
#Hot press for 5 minutes.  
##Enumerate each of those.
#Remove and let cool.
 
==Notes==
#List troubleshooting tips here. 
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
#Anecdotal observations that might be of use to others can also be posted here. 
 
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
 
==References==
'''Relevant papers and books'''
<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. -->
<biblio>
#Goldbeter-PNAS-1981 pmid=6947258
#Jacob-JMB-1961 pmid=13718526
#Ptashne-Genetic-Switch isbn=0879697164
</biblio>
 
==Contact==
*Who has experience with this protocol?
 
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].  
 
<!-- You can tag this protocol with various categories.  See the [[Categories]] page for more information. -->
[[Category:Protocol]]
[[Category:Needs attention]]  <!--Delete this line once the protocol is complete-->
 
<!-- Move the relevant categories above this line to tag your protocol with the label
[[Category:In vitro]]
 
[[Category:In vivo]]
 
[[Category:In silico]]
 
[[Category:DNA]]
 
[[Category:RNA]]
 
[[Category:Protein]]
 
[[Category:Chemical]]
 
[[Category:Escherichia coli]]
 
[[Category:Yeast]]
-->

Revision as of 13:01, 2 November 2007

This page is a template and should not be edited.
Click here, copy the source, and paste it into your page.



Interested in posting a protocol on OpenWetWare? Here is a template to help you do so.

Click the view source tab and copy everything below this line. Paste it into your new protocol page. Then replace the text in this page with your own protocol. Feel free to add or delete sections as appropriate.

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • supply 1 (i.e. tubes of a certain size? spreaders?)
  • reagent 1
  • X μL reagent 2
    • component A (reagent 2 is made up of multiple components)
    • component B
  • equipment 1
  • equipment 2

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 | PubMed ID:6947258 | HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 | PubMed ID:13718526 | HubMed [Jacob-JMB-1961]
  3. ISBN:0879697164 [Ptashne-Genetic-Switch]

All Medline abstracts: PubMed | HubMed

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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