Protocols/Template: Difference between revisions
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==Overview== | ==Overview== | ||
This protocol describes the procedure for streaking bacteria on an Agar plate. | |||
==Materials== | ==Materials== | ||
*Agar plate(s) | |||
*Bacteria sample from old plate | |||
* | *Inoculation loop | ||
* | *Bunsen Burner | ||
*70% Ethanol | |||
* | *Parafilm | ||
* | |||
* | |||
* | |||
==Procedure== | ==Procedure== | ||
# | #Wipe down the bench with 70% EtOH and start a Bunsen burner flame. | ||
#Step | #Label new agar plate(s), on the bottom, with your initials, the date and strain of bacteria, and prepare plastic beaker for containing the disposable at the side#Step 3 | ||
# | #Flame until red to disinfect the inoculation loop, from top toward the loop, and allow it to cool down by inserting to loop into the agar at a corner of the new agar plate. | ||
# | #(work by the flame) Open the old streaking plate, wipe down water vapor on the cover with Kimwipes, and take one (1) pure culture with the loop. | ||
## | #Slide the loop over the new agar plate. First start circulating ~10 times at a corner of the streaking area, followed by moving the loop in zigzag patterns to spread the colony on all 4 quadrants over the plate. Between each spread, flame the loop to disinfect and allow it to cool down. Cross over the previous quadrant 2~3 times and avoid cross over the previous quadrant more than once or into any other quadrants for the last streak. | ||
# | #Cover the new dish and seal the edge of the old plate with parafilm. | ||
#Incubate the new plate @37oC in a upside-down position (to allow water to drip onto the cover) overnight (~24hrs) | |||
#Bleach and wipe down the bench with 70% EtOH again at the end. | |||
#Take the plate(s) out from the incubator on the next day and store it inside the fridge with parafilm sealing the edge. (good for a month) | |||
#Bleach the old dish (entirely cover the plate) and seal with parafilm. Dispose it in the biohazard trash. | |||
==Notes== | ==Notes== | ||
==References== | ==References== | ||
==Contact== | ==Contact== | ||
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or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | ||
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Revision as of 15:33, 11 February 2008
| This page is a template and should not be edited. Click here, copy the source, and paste it into your page. |
Overview
This protocol describes the procedure for streaking bacteria on an Agar plate.
Materials
- Agar plate(s)
- Bacteria sample from old plate
- Inoculation loop
- Bunsen Burner
- 70% Ethanol
- Parafilm
Procedure
- Wipe down the bench with 70% EtOH and start a Bunsen burner flame.
- Label new agar plate(s), on the bottom, with your initials, the date and strain of bacteria, and prepare plastic beaker for containing the disposable at the side#Step 3
- Flame until red to disinfect the inoculation loop, from top toward the loop, and allow it to cool down by inserting to loop into the agar at a corner of the new agar plate.
- (work by the flame) Open the old streaking plate, wipe down water vapor on the cover with Kimwipes, and take one (1) pure culture with the loop.
- Slide the loop over the new agar plate. First start circulating ~10 times at a corner of the streaking area, followed by moving the loop in zigzag patterns to spread the colony on all 4 quadrants over the plate. Between each spread, flame the loop to disinfect and allow it to cool down. Cross over the previous quadrant 2~3 times and avoid cross over the previous quadrant more than once or into any other quadrants for the last streak.
- Cover the new dish and seal the edge of the old plate with parafilm.
- Incubate the new plate @37oC in a upside-down position (to allow water to drip onto the cover) overnight (~24hrs)
- Bleach and wipe down the bench with 70% EtOH again at the end.
- Take the plate(s) out from the incubator on the next day and store it inside the fridge with parafilm sealing the edge. (good for a month)
- Bleach the old dish (entirely cover the plate) and seal with parafilm. Dispose it in the biohazard trash.
Notes
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
