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| ==Procedure== | | ==Procedure== |
| #Wipe down the bench with 70% EtOH and start a Bunsen burner flame. | | #Step1 |
| #Label new agar plate(s), on the bottom, with your initials, the date and strain of bacteria, and prepare plastic beaker for containing the disposable at the side#Step 3 | | #Step2 |
| #Flame until red to disinfect the inoculation loop, from top toward the loop, and allow it to cool down by inserting to loop into the agar at a corner of the new agar plate.
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| #(work by the flame) Open the old streaking plate, wipe down water vapor on the cover with Kimwipes, and take one (1) pure culture with the loop.
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| #Slide the loop over the new agar plate. First start circulating ~10 times at a corner of the streaking area, followed by moving the loop in zigzag patterns to spread the colony on all 4 quadrants over the plate. Between each spread, flame the loop to disinfect and allow it to cool down. Cross over the previous quadrant 2~3 times and avoid cross over the previous quadrant more than once or into any other quadrants for the last streak.
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| #Cover the new dish and seal the edge of the old plate with parafilm.
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| #Incubate the new plate @37oC in a upside-down position (to allow water to drip onto the cover) overnight (~24hrs)
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| #Bleach and wipe down the bench with 70% EtOH again at the end.
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| #Take the plate(s) out from the incubator on the next day and store it inside the fridge with parafilm sealing the edge. (good for a month)
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| #Bleach the old dish (entirely cover the plate) and seal with parafilm. Dispose it in the biohazard trash.
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| ==Notes== | | ==Notes== |
Revision as of 15:48, 11 February 2008
Overview
Materials
Procedure
- Step1
- Step2
Notes
References
Contact
Sylvanus.
or instead, [[Talk:Template:Sylvanus Y.K. Lee|discuss this protocol]].
