Protocols/Template: Difference between revisions
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'''Interested in posting a protocol on OpenWetWare? Here is a template to help you do so.''' | '''Interested in posting a protocol on OpenWetWare? Here is a template to help you do so.''' | ||
'''Click the edit tab and copy everything below this line. Paste it into your new protocol page.''' | '''Click the edit tab and copy everything below this line. Paste it into your new protocol page. Then replace the text in this page with your own protocol. Feel free to add or delete sections as appropriate.''' | ||
<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --> | |||
==Overview== | ==Overview== | ||
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==References== | ==References== | ||
If this protocol has papers or books associated with it, list those references here. | '''Relevant papers and books''' | ||
<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. --> | |||
<biblio> | |||
#Goldbeter-PNAS-1981 pmid=6947258 | |||
#Jacob-JMB-1961 pmid=13718526 | |||
#Ptashne-Genetic-Switch isbn=0879697164 | |||
</biblio> | |||
Revision as of 08:10, 1 June 2006
Interested in posting a protocol on OpenWetWare? Here is a template to help you do so.
Click the edit tab and copy everything below this line. Paste it into your new protocol page. Then replace the text in this page with your own protocol. Feel free to add or delete sections as appropriate.
Overview
Replace this sentence with a brief description of the protocol and its goal.
Materials
List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.
- supply 1 (i.e. tubes of a certain size? spreaders?)
- reagent 1
- X μL reagent 2
- component A (reagent 2 is made up of multiple components)
- component B
- equipment 1
- equipment 2
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
