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=Juncker: fluorosilane coated glass slide =
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== Overview ==
How to prepare a fluorosilane coated glass slide. This coating is useful if hydrophobic surfaces are needed.<br>
Author: [[Benjamin Robledo]] <br>
Date: June 18, 2007 <br>
Version: 1.7<br>


== Materials ==


Reagents:
*Ethanol and acetone staining dishes.
*A couple of drops of [[Internal:Chemicals|Trichloro(1H, 1H, 2H, 2H - Perfluorooctyl)Silane, 97%]].
*Distilled water.


Equipment:
*[[Internal:Cover glass|Glass slides]] to be coated.
*2 [[Internal:Petri dishes|Petri dishes]].
*A set of [[Internal:Tweezers|tweezers]]
*Diamond-tip engraving pen
*Ultrasonic Bath.
*Nitrogen gun.
*Plasma chamber.
*Vaccum chamber.


'''Interested in posting a protocol on OpenWetWare?  Here is a template to help you do so.''' 
== Procedure ==


'''Click the view source tab and copy everything below this line. Paste it into your new protocol page. Then replace the text in this page with your own protocol.  Feel free to add or delete sections as appropriate.'''
# Clean the glass slides to be coated following the [[Internal:Protocol Ultrasonic Bath|Ultrasonic Bath]] protocol. <br> [[Mateu Pla-Roca|Mateu says]]  13:55, 17 June 2007 (EDT)''': In order to know which is the good side of your sample, write and '''R''' on the '''R'''ear of the slide with a diamond-tip engraving pen.
[[Category:Template]]
# Bring the petri dish with the slides to the plasma chamber located in room B-2. Place the petri dish with the slides inside the plasma chamber for 30 seconds. <br> [[Benjamin_Robledo|Ben says]] 14:10, 6 June 2007 (EDT)''': Make sure you know how to operate the plasma chamber before using it. <br>
<!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! -->
# Place the petri dish with the slides on the vacuum chamber labeled "SILANES" along with another petri dish containing a 50 microl iters of Trichloro(1H, 1H, 2H, 2H - Perfluorooctyl) Silane, 97%.<br> [[Mateu Pla-Roca|Mateu says]] 14:17, 17 June 2007 (EDT)''': Use a  pipette to transfer amount of the silane to the petri dish. After the transfer remove immediately the tip from the pipette in order to protect the pipette from the silane vapor
# Turn on the vaccum pump for 5 minutes. Turn pump off a leave slides on vaccum for 30 minutes.
# Open vacuum chamber
# Put 3 drops of water on the petri dish that contains the silane in order to neutralize it
# Rinse the plastic petri dish with ethanol and dispose the silane-ethanol solution on the plastic bucket labelled "COMMON ORGANIC SOLVENTS".
# Your newly coated slides are now ready to use!! enjoy!!
# You can store your surfaces for long time. Protect your surface from the dust.


==Overview==
== References ==


Replace this sentence with a brief description of the protocol and its goal.
== Contact ==


==Materials==
[[Mateu Pla-Roca]] knows all the tricks behind this protocol.
List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.


*supply 1 (i.e. tubes of a certain size? spreaders?)
[[Category:Protocols]]
*reagent 1
*X &mu;L reagent 2
**component A (reagent 2 is made up of multiple components)
**component B
*equipment 1
*equipment 2
 
==Procedure==
#Step 1
#Step 2
#*Step 2 has some additional information that goes with it.  i.e. Keep at 4&deg;C.
#Step 3
##Step 3 has multiple sub-steps within it.
##Enumerate each of those.
 
==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
#List troubleshooting tips here. 
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
#Anecdotal observations that might be of use to others can also be posted here. 
 
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
 
==References==
'''Relevant papers and books'''
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<biblio>
#Goldbeter-PNAS-1981 pmid=6947258
#Jacob-JMB-1961 pmid=13718526
#Ptashne-Genetic-Switch isbn=0879697164
</biblio>
 
==Contact==
*Who has experience with this protocol?
 
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].
 
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Revision as of 15:21, 25 November 2008

Juncker: fluorosilane coated glass slide

Overview

How to prepare a fluorosilane coated glass slide. This coating is useful if hydrophobic surfaces are needed.
Author: Benjamin Robledo
Date: June 18, 2007
Version: 1.7

Materials

Reagents:

Equipment:

Procedure

  1. Clean the glass slides to be coated following the Ultrasonic Bath protocol.
    Mateu says 13:55, 17 June 2007 (EDT): In order to know which is the good side of your sample, write and R on the Rear of the slide with a diamond-tip engraving pen.
  2. Bring the petri dish with the slides to the plasma chamber located in room B-2. Place the petri dish with the slides inside the plasma chamber for 30 seconds.
    Ben says 14:10, 6 June 2007 (EDT): Make sure you know how to operate the plasma chamber before using it.
  3. Place the petri dish with the slides on the vacuum chamber labeled "SILANES" along with another petri dish containing a 50 microl iters of Trichloro(1H, 1H, 2H, 2H - Perfluorooctyl) Silane, 97%.
    Mateu says 14:17, 17 June 2007 (EDT): Use a pipette to transfer amount of the silane to the petri dish. After the transfer remove immediately the tip from the pipette in order to protect the pipette from the silane vapor
  4. Turn on the vaccum pump for 5 minutes. Turn pump off a leave slides on vaccum for 30 minutes.
  5. Open vacuum chamber
  6. Put 3 drops of water on the petri dish that contains the silane in order to neutralize it
  7. Rinse the plastic petri dish with ethanol and dispose the silane-ethanol solution on the plastic bucket labelled "COMMON ORGANIC SOLVENTS".
  8. Your newly coated slides are now ready to use!! enjoy!!
  9. You can store your surfaces for long time. Protect your surface from the dust.

References

Contact

Mateu Pla-Roca knows all the tricks behind this protocol.

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