Protocols v.4: Difference between revisions
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==''In vivo''== | ==''In vivo''== | ||
{{size|3|'''''Escherichia coli'''''}} | {{size|3|'''''Escherichia coli'''''}} {{hide|1= | ||
*[[Bacterial cell culture]] | *[[Bacterial cell culture]] | ||
*[[Bacterial Transformation | Bacterial transformation]] | *[[Bacterial Transformation | Bacterial transformation]] | ||
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*[http://www.cf.ac.uk/biosi/staff/ehrmann/tools/factors.htm Protein expression resource] | *[http://www.cf.ac.uk/biosi/staff/ehrmann/tools/factors.htm Protein expression resource] | ||
*[[S_N Chemostat Protocol]] | *[[S_N Chemostat Protocol]] | ||
}} | |||
{{size|3|'''''Mesoplasma florum'''''}} | |||
{{size|3|'''''Mesoplasma florum'''''}} {{hide|1= | |||
*[[Mesoplama florum:Culture media|Preparing culture media]] | *[[Mesoplama florum:Culture media|Preparing culture media]] | ||
}} | |||
{{size|3|'''T7'''}} | |||
{{size|3|'''T7'''}} {{hide|1= | |||
*[[Cesium Chloride Purification of T7]] | *[[Cesium Chloride Purification of T7]] | ||
*[[Studier Lysate Prep|Simple lysate protocol from plaque or lysate]] | *[[Studier Lysate Prep|Simple lysate protocol from plaque or lysate]] | ||
}} | |||
{{size|3|'''Yeast'''}} | |||
{{size|3|'''Yeast'''}} {{hide|1= | |||
*[[Assaying mating]] | *[[Assaying mating]] | ||
*[[Cell Cycle Arrest]] | *[[Cell Cycle Arrest]] | ||
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*[[Yeast DNA Prep]] | *[[Yeast DNA Prep]] | ||
}} | |||
</div> | </div> | ||
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==''In vitro''== | ==''In vitro''== | ||
{{size|3|'''Nucleic acids'''}} | {{size|3|'''Nucleic acids'''}} {{hide|1= | ||
*[[Nucleic acid precipitation]] | *[[Nucleic acid precipitation]] | ||
*[[Phenol/chloroform extraction]] | *[[Phenol/chloroform extraction]] | ||
}} | |||
{{size|3|'''DNA'''}} | |||
{{size|3|'''DNA'''}} {{hide|1= | |||
*[[Agarose gel electrophoresis]] | *[[Agarose gel electrophoresis]] | ||
*[[Annealing Primers]] | *[[Annealing Primers]] | ||
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*[[Site-directed mutagenesis]] | *[[Site-directed mutagenesis]] | ||
*[[Templiphi]] | *[[Templiphi]] | ||
}} | |||
{{size|3|'''RNA'''}} | |||
{{size|3|'''RNA'''}} {{hide|1= | |||
*[[In vitro transcription with T7 RNA polymerase]] | *[[In vitro transcription with T7 RNA polymerase]] | ||
*[[Northern Blot, 32P End-Labeled Probes |Northern Blot, <sup>32</sup>P End-Labeled Probes]] | *[[Northern Blot, 32P End-Labeled Probes |Northern Blot, <sup>32</sup>P End-Labeled Probes]] | ||
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*[[RNA Extraction]] | *[[RNA Extraction]] | ||
*[[RNase Protection Assay]] | *[[RNase Protection Assay]] | ||
}} | |||
{{size|3|'''Protein'''}} | |||
{{size|3|'''Protein'''}} {{hide|1= | |||
*[[Acrylamide Gels|Acrylamide gels and SDS-PAGE]] | *[[Acrylamide Gels|Acrylamide gels and SDS-PAGE]] | ||
*[[Silver: Destain|Coomassie Destain]] | *[[Silver: Destain|Coomassie Destain]] | ||
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*[[Western Blot]] | *[[Western Blot]] | ||
*[[Purification of His-tagged proteins]] | *[[Purification of His-tagged proteins]] | ||
}} | |||
</div> | </div> | ||
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==''In silico''== | ==''In silico''== | ||
{{size|3|'''Sequence Analysis'''}} | {{size|3|'''Sequence Analysis'''}} {{hide|1= | ||
*[[BLAST]] | *[[BLAST]] | ||
*[[CLUSTALW]] | *[[CLUSTALW]] | ||
}} | |||
{{size|3|'''Structure'''}} | |||
{{size|3|'''Structure'''}} {{hide|1= | |||
*[[THREADER]] | *[[THREADER]] | ||
*[[Homology Modelling]] | *[[Homology Modelling]] | ||
}} | |||
{{size|3|'''Pathway Modelling'''}} | |||
{{size|3|'''Pathway Modelling'''}} {{hide|1= | |||
*[[FBA|Flux Balance Analysis]] | *[[FBA|Flux Balance Analysis]] | ||
*[[MoMA|Minimisation of Metabolic Adjustment]] | *[[MoMA|Minimisation of Metabolic Adjustment]] | ||
}} | |||
==Other== | ==Other== | ||
{{size|3|'''Flow Cytometry'''}} | {{size|3|'''Flow Cytometry'''}} {{hide|1= | ||
*[[Flow Calibration]] | *[[Flow Calibration]] | ||
}} | |||
{{size|3|'''Microfluidics'''}} | |||
{{size|3|'''Microfluidics'''}} {{hide|1= | |||
*[[Design a Microfluidic Chip]] | *[[Design a Microfluidic Chip]] | ||
*[[Order a Microfluidic Chip from the Foundry]] | *[[Order a Microfluidic Chip from the Foundry]] | ||
*[[Operate Microfluidic Chemostat]] | *[[Operate Microfluidic Chemostat]] | ||
}} | |||
{{size|3|'''Microscopy'''}} | |||
{{size|3|'''Microscopy'''}} {{hide|1= | |||
*[[Agarose Pads for Microscopy]] | *[[Agarose Pads for Microscopy]] | ||
*[[Quantitative Microscopy]] | *[[Quantitative Microscopy]] | ||
*[[Silver: Time-Lapse Microscopy|Time-Lapse Microscopy]] | *[[Silver: Time-Lapse Microscopy|Time-Lapse Microscopy]] | ||
}} | |||
{{size|3|'''Plate reader'''}} | |||
{{size|3|'''Plate reader'''}} {{hide|1= | |||
*[[Endy:Victor3 plate reader| Perkin Elmer Victor<sup>3</sup>]]<br>-Plate reader usage protocols | *[[Endy:Victor3 plate reader| Perkin Elmer Victor<sup>3</sup>]]<br>-Plate reader usage protocols | ||
}} | |||
{{size|3|'''General resources'''}} | {{size|3|'''General resources'''}} | ||
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|} | |} | ||
{{show hide all}} | |||
__NOTOC__ | __NOTOC__ | ||
Latest revision as of 18:09, 11 September 2007
In vivoEscherichia coli
|
In vitroNucleic acids
|
In silicoSequence Analysis
OtherFlow Cytometry
General resources
Protocols in Development Please Contribute! |
[show all] [hide all]
