Protocols v.4

In vivo Escherichia coli [show][hide] Bacterial cell culture Bacterial transformation Preparing chemically competent cells (TSS) Transforming chemically competent cells (TSS) Preparing electrocompetent cells Transforming electrocompetent cells β-Galactosidase Assay (A better Miller) Colony PCR Making a long term stock of bacteria Miniprep P1vir phage transduction Pour LB plates Protein expression resource S_N Chemostat Protocol Mesoplasma florum [show][hide] Preparing culture media T7 [show][hide] Cesium Chloride Purification of T7 Simple lysate protocol from plaque or lysate Yeast [show][hide] Assaying mating Cell Cycle Arrest Dropout plates for yeast High Efficiency Transformation Layered plates Pour YPD plates Simple Yeast Lysate (for Western etc.) Time-Lapse Microscopy Yeast Colony PCR Yeast DNA Prep

In vitro Nucleic acids [show][hide] Nucleic acid precipitation Phenol/chloroform extraction DNA [show][hide] Agarose gel electrophoresis Annealing Primers Designing primers DNA Ligation Library Generation Miniprep PCR Phosphatase treatment of linearized vector Polony Protocols (Church/Mitra Lab) PNK Treatment of DNA Ends Purification of DNA Reconstituting primers Restriction digest 'Round-the-horn site-directed mutagenesis Sequencing DNA Site-directed mutagenesis Templiphi RNA [show][hide] In vitro transcription with T7 RNA polymerase Northern Blot, 32P End-Labeled Probes Probe Prep, 32P End-Labeled Probes RNA Extraction RNase Protection Assay Protein [show][hide] Acrylamide gels and SDS-PAGE Coomassie Destain Coomassie Stain HPLC Silver staining Western Blot Purification of His-tagged proteins

In silico Sequence Analysis [show][hide] BLAST CLUSTALW Structure [show][hide] THREADER Homology Modelling Pathway Modelling [show][hide] Flux Balance Analysis Minimisation of Metabolic Adjustment Other Flow Cytometry [show][hide] Flow Calibration Microfluidics [show][hide] Design a Microfluidic Chip Order a Microfluidic Chip from the Foundry Operate Microfluidic Chemostat Microscopy [show][hide] Agarose Pads for Microscopy Quantitative Microscopy Time-Lapse Microscopy Plate reader [show][hide] Perkin Elmer Victor3 -Plate reader usage protocols General resources Protocol-online- Contains a lot of useful protocols and a popular discussion section. Protocols in Development Please Contribute! Pulse-chase protein production Fixing cells B-galactosidase assay RNA Half-life Denaturing acrylamide gel purification of nucleic acids Quantification of nucleic acids

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