Purification of DNA

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There are several methods for purifying DNA.  The one you choose depends on the nature of your DNA sample and your downstream applications.
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#[[Ethanol precipitation of small DNA fragments]]
#[[Ethanol precipitation of small DNA fragments]]
#[[Micropure EZ and Microcon purification]]
#[[Micropure EZ and Microcon purification]]
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#*for small DNA fragments (~50-200 bp in length)
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#*removes proteins, nucleotides and salts
#[[Miniprep]]
#[[Miniprep]]
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#*for purifying plasmid DNA from ''E. coli'' cells
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#[[QIAquick PCR purification]]
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#*for typical DNA fragments (> 200 bp in length)

Revision as of 14:44, 31 May 2005

There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.

  1. Ethanol precipitation of small DNA fragments
  2. Micropure EZ and Microcon purification
    • for small DNA fragments (~50-200 bp in length)
    • removes proteins, nucleotides and salts
  3. Miniprep
    • for purifying plasmid DNA from E. coli cells
  4. QIAquick PCR purification
    • for typical DNA fragments (> 200 bp in length)
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