Purification of DNA: Difference between revisions
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#[[Ethanol precipitation of small DNA fragments]] | #[[Ethanol precipitation of small DNA fragments]] | ||
#[[ | #[[Centrifugal filtration/Nucleic acids]] | ||
#*for small DNA fragments (~50-200 bp in length) | #*for small DNA fragments (~50-200 bp in length) | ||
#*removes proteins, nucleotides and salts | #*removes proteins, nucleotides and salts | ||
Revision as of 20:58, 13 September 2006
There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.
- Ethanol precipitation of small DNA fragments
- Centrifugal filtration/Nucleic acids
- for small DNA fragments (~50-200 bp in length)
- removes proteins, nucleotides and salts
- Miniprep
- for purifying plasmid DNA from E. coli cells
- QIAquick PCR purification
- for typical DNA fragments (> 200 bp in length)
