Purification of DNA
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#[[QIAquick PCR purification]] | #[[QIAquick PCR purification]] | ||
#*for typical DNA fragments (> 200 bp in length) | #*for typical DNA fragments (> 200 bp in length) | ||
| - | #[[Isopropanol Precipitation for PCR Purification | + | #[[Isopropanol Precipitation for PCR Purification]] |
#*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns | #*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns | ||
Revision as of 14:49, 8 December 2006
There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.
- Ethanol precipitation of small DNA fragments
- Centrifugal filtration/Nucleic acids
- for small DNA fragments (~50-200 bp in length)
- removes proteins, nucleotides and salts
- Miniprep
- for purifying plasmid DNA from E. coli cells
- QIAquick PCR purification
- for typical DNA fragments (> 200 bp in length)
- Isopropanol Precipitation for PCR Purification
- use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns
