Q-PCR

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'''Quantitative PCR''' (Q-PCR or qPCR) is used to quantify starting amounts of nucleic acid template.  In quantitative PCR, the DNA amounts can be measured either at the end of the PCR step (end-point assay) or during the PCR step ([[Real-time PCR|real-time PCR]]).  Today [[Real-time PCR|real-time PCR]] is more commonly used because it can be more precise.
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{{back to protocols}}
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{{dablink|[[QRT-PCR|quantitative reverse transcription PCR]]}}
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Frequently, quantitative PCR is used to measure RNA levels (although it can also be used to measure DNA levels)[[RNA extraction|Isolated RNA]] is reverse transcribed into cDNAThen quantitative PCR is run on the cDNA sample.  This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.
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'''Quantitative PCR''' (Q-PCR or qPCR) is a [[PCR techniques|PCR technique]] used to quantify starting amounts of DNA templateAmounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps ([[Real-time PCR|real-time PCR]]).  Today [[Real-time PCR|real-time PCR]] is more commonly used because it can be more precise.
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== See also ==
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Frequently, quantitative PCR is used to measure RNA instead of DNA levels. To do this, [[RNA extraction|isolated RNA]] is reverse transcribed into cDNA.  Then quantitative PCR is run on the cDNA sample.  This sub-technique is called quantitative reverse transcriptase PCR ([[qRT-PCR]]) or even longer names.
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* [[PCR]], [[PCR techniques]] and [[qRT-PCR]] on OpenWetWare.
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* An excellent, detailed [http://pathmicro.med.sc.edu/pcr/realtime-home.htm Q-PCR tutorial] by Margaret and Richard Hunt, University of South Carolina
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==See also==
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* [[Real-time PCR]] and [[qRT-PCR|quantitative reverse transcriptase PCR]]
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* [[PCR]] and [[PCR techniques]]
* Wikipedia entry on [[wikipedia:Quantitative polymerase chain reaction|Q-PCR]]
* Wikipedia entry on [[wikipedia:Quantitative polymerase chain reaction|Q-PCR]]
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* The venerable [http://tech.groups.yahoo.com/group/qpcrlistserver/ qpcrlistserv]. Anyone doing qPCR should be subscribed to this list.
 
[[Category:Protocol]]  
[[Category:Protocol]]  

Current revision

back to protocols

Quantitative PCR (Q-PCR or qPCR) is a PCR technique used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps (real-time PCR). Today real-time PCR is more commonly used because it can be more precise.

Frequently, quantitative PCR is used to measure RNA instead of DNA levels. To do this, isolated RNA is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcriptase PCR (qRT-PCR) or even longer names.

See also

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