QIAquick PCR purification: Difference between revisions

Latest revision as of 10:38, 9 February 2007

Anecdotal evidence suggests that you can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA more time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of Meagan Lizarazo.

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 [http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual]
-==Tips==
+==Notes==
-You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge).  This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter.  Courtesy of [[User:Meaganl|Meagan Lizarazzo]].
+*Anecdotal evidence suggests that you can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge).  This gives the DNA more time to bind to the filter and hence reduces the amount of DNA that get washed through the filter.  Courtesy of [[User:Meaganl|Meagan Lizarazo]].