QIAquick PCR purification

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[http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual]
[http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual]
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==Tips==
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==Notes==
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You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge).  This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter.  Courtesy of [[User:Meaganl|Meagan Lizarazo]].
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*Anecdotal evidence suggests that you can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge).  This gives the DNA more time to bind to the filter and hence reduces the amount of DNA that get washed through the filter.  Courtesy of [[User:Meaganl|Meagan Lizarazo]].

Current revision

Kit manual

Notes

  • Anecdotal evidence suggests that you can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA more time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of Meagan Lizarazo.
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