QIAquick PCR purification: Difference between revisions
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[http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual] | [http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual] | ||
== | ==Notes== | ||
You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl|Meagan Lizarazo]]. | *You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl|Meagan Lizarazo]]. | ||
Revision as of 10:15, 9 February 2007
Notes
- You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of Meagan Lizarazo.
