QIAquick PCR purification

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Revision as of 12:20, 9 February 2007 by Barry Canton (Talk | contribs)
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Kit manual


  • You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA more time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of Meagan Lizarazo.
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