QIAquick PCR purification - Revision history

Barry Canton at 17:38, 9 February 2007

Barry Canton — Fri, 09 Feb 2007 17:38:19 GMT

←Older revision		Revision as of 17:38, 9 February 2007
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	==Notes==		==Notes==
-	*~~You~~ can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA more time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan Lizarazo]].	+	*Anecdotal evidence suggests that you can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA more time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan Lizarazo]].

Barry Canton: /* Notes */

Barry Canton — Fri, 09 Feb 2007 17:20:21 GMT

Notes

←Older revision		Revision as of 17:20, 9 February 2007
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	==Notes==		==Notes==
-	*You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan Lizarazo]].	+	*You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA more time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan Lizarazo]].

Barry Canton at 17:15, 9 February 2007

Barry Canton — Fri, 09 Feb 2007 17:15:35 GMT

←Older revision		Revision as of 17:15, 9 February 2007
Line 1:		Line 1:
	[http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual]		[http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual]

-	==~~Tips~~==	+	==Notes==
-	You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan Lizarazo]].	+	*You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan Lizarazo]].

Barry Canton at 17:15, 9 February 2007

Barry Canton — Fri, 09 Feb 2007 17:15:17 GMT

←Older revision		Revision as of 17:15, 9 February 2007
Line 2:		Line 2:

	==Tips==		==Tips==
-	You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan ~~Lizarazzo~~]].	+	You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan Lizarazo]].

Barry Canton at 17:15, 9 February 2007

Barry Canton — Fri, 09 Feb 2007 17:15:02 GMT

←Older revision		Revision as of 17:15, 9 February 2007
Line 2:		Line 2:

	==Tips==		==Tips==
-	You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl]].	+	You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl\|Meagan Lizarazzo]].

Barry Canton: New page: [http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual] ==Tips== You can obtain a higher yield by doing the first spi...

Barry Canton — Fri, 09 Feb 2007 17:14:47 GMT

New page: [http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual] ==Tips== You can obtain a higher yield by doing the first spi...

New page

[http://www1.qiagen.com/literature/handbooks/PDF/DNACleanupAndConcentration/QQ_Spin/1021422_HBQQSpin_072002WW.pdf Kit manual]

==Tips==
You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of [[User:Meaganl]].