QIAquick PCR purification

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Revision as of 13:15, 9 February 2007 by Barry Canton (Talk | contribs)
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Kit manual


You can obtain a higher yield by doing the first spin (of buffer PB1) at a reduced speed (~50% of max. on a typical tabletop centrifuge). This gives the DNA time to bind to the filter and hence reduces the amount of DNA that get washed through the filter. Courtesy of Meagan Lizarazzo.

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